Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4035710..4036385 Replicon chromosome
Accession NZ_CP021749
Organism Enterobacter cloacae complex sp. strain AR_0163

Toxin (Protein)


Gene name higB Uniprot ID A0A7T9SGM4
Locus tag AM409_RS20055 Protein ID WP_032654718.1
Coordinates 4036086..4036385 (-) Length 100 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A2J0PXC9
Locus tag AM409_RS20050 Protein ID WP_015571639.1
Coordinates 4035710..4036075 (-) Length 122 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM409_RS20030 (AM409_19935) 4031404..4032783 + 1380 WP_045911566.1 amidohydrolase family protein -
AM409_RS20035 (AM409_19940) 4032894..4033496 - 603 WP_017382759.1 short chain dehydrogenase -
AM409_RS20040 (AM409_19945) 4033594..4034481 + 888 WP_015571641.1 LysR family transcriptional regulator -
AM409_RS20045 (AM409_19950) 4034502..4035683 + 1182 WP_017382758.1 PLP-dependent aminotransferase family protein -
AM409_RS20050 (AM409_19955) 4035710..4036075 - 366 WP_015571639.1 HigA family addiction module antitoxin Antitoxin
AM409_RS20055 (AM409_19960) 4036086..4036385 - 300 WP_032654718.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AM409_RS20060 (AM409_19965) 4036636..4037835 - 1200 WP_017382757.1 HlyD family efflux transporter periplasmic adaptor subunit -
AM409_RS20065 (AM409_19970) 4037816..4040008 - 2193 WP_045328302.1 type I secretion system permease/ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-95)

Antitoxin

Cro/C1-type helix-turn-helix domain

(25-72)


Sequences

Toxin        


Download         Length: 100 a.a.        Molecular weight: 11582.25 Da        Isoelectric Point: 9.8425

>T77289 WP_032654718.1 NZ_CP021749:c4036385-4036086 [Enterobacter cloacae complex sp.]
MINHYRDQWLEDFFLYGRSSNVIPLNLETALARKLDIINAAMSHLDLRSPPGNMYEALSPPLKGYSSIRVNRQYRLVFRW
SAGKADDLYLSPHKYTQHK

Download         Length: 300 bp

>T77289 NZ_CP021749:c4036385-4036086 [Enterobacter cloacae complex sp.]
ATGATTAATCATTATCGGGATCAATGGCTTGAGGATTTTTTTCTCTACGGGAGATCGAGCAATGTTATTCCTTTGAATCT
GGAAACAGCGCTTGCAAGAAAGCTCGACATCATCAATGCGGCCATGTCGCACCTGGATTTACGATCGCCACCGGGCAATA
TGTATGAAGCATTGAGTCCTCCGTTGAAGGGATATTCCTCTATCCGGGTAAACAGACAATACAGGCTTGTATTTCGCTGG
TCAGCGGGAAAAGCAGACGATCTCTATCTCTCTCCACACAAGTACACGCAACACAAGTGA

Antitoxin


Download         Length: 122 a.a.        Molecular weight: 13705.73 Da        Isoelectric Point: 9.5936

>AT77289 WP_015571639.1 NZ_CP021749:c4036075-4035710 [Enterobacter cloacae complex sp.]
MTLQQALRKPTTPGEVLQYEYLEPLNLKINDLAEMLNVHRNTVSALVNNNRKLTADMAIKLAKAFNTTIEFWLNLQLNVD
IWEAQSNSRTQEELSRIKTVAEVMAKRKSGKPDVTLHGNSA

Download         Length: 366 bp

>AT77289 NZ_CP021749:c4036075-4035710 [Enterobacter cloacae complex sp.]
ATGACACTTCAACAGGCACTCCGCAAACCCACCACGCCGGGCGAGGTGTTGCAGTATGAGTATCTTGAACCGCTCAATCT
GAAAATCAACGATCTGGCAGAGATGCTAAATGTACACCGCAATACCGTAAGCGCATTGGTCAATAACAATCGCAAACTTA
CTGCCGATATGGCGATCAAACTGGCAAAAGCCTTTAATACCACTATTGAATTTTGGCTGAACTTACAGCTAAACGTTGAT
ATCTGGGAAGCGCAATCTAACTCCCGCACGCAGGAGGAGTTAAGCCGGATAAAAACCGTTGCGGAAGTCATGGCGAAGCG
AAAATCCGGCAAGCCAGACGTTACCCTGCACGGGAACAGCGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7T9SGM4

Antitoxin

Source ID Structure
AlphaFold DB A0A2J0PXC9

References