Detailed information of TA system TA077259

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 4919..5183
• Replicon: plasmid tig00002897alt
• Accession: NZ_CP021739
• Organism: Escherichia coli strain AR_0150

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: AM397_RS26105
• Protein ID: WP_001387489.1
• Coordinates: 5031..5183 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	4919..4979 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM397_RS26065	1044..1307	+	264	WP_032180564.1	ash family protein	-
AM397_RS26070	1225..1512	+	288	WP_000074855.1	conjugal transfer protein TraA	-
AM397_RS26080	1954..2259	+	306	Protein_3	transcription termination factor NusG	-
AM397_RS26085	2273..2970	-	698	Protein_4	IS1 family transposase	-
AM397_RS26095	3026..4183	-	1158	Protein_5	IS1380-like element ISEc9 family transposase	-
AM397_RS26790	4369..4716	-	348	Protein_6	protein finQ	-
-	4919..4979	-	61	NuclAT_0	-	Antitoxin
-	4919..4979	-	61	NuclAT_0	-	Antitoxin
-	4919..4979	-	61	NuclAT_0	-	Antitoxin
-	4919..4979	-	61	NuclAT_0	-	Antitoxin
AM397_RS26105	5031..5183	+	153	WP_001387489.1	Hok/Gef family protein	Toxin
AM397_RS26110	5255..5506	-	252	WP_001291964.1	hypothetical protein	-
AM397_RS26115	5806..6102	+	297	WP_011264046.1	hypothetical protein	-
AM397_RS27010	6167..6343	-	177	WP_001054897.1	hypothetical protein	-
AM397_RS26120	6735..6944	+	210	WP_000062603.1	HEAT repeat domain-containing protein	-
AM397_RS26130	7169..7866	+	698	Protein_12	IS1 family transposase	-
AM397_RS26135	8319..9464	+	1146	WP_039023136.1	class C extended-spectrum beta-lactamase CMY-42	-
AM397_RS26140	9558..10091	+	534	WP_001221666.1	lipocalin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCMY-42 / blaTEM-1B	-	1..50235	50235
	-	inside	IScluster/Tn	blaCMY-42	-	2695..9464	6769

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T77259 WP_001387489.1 NZ_CP021739:5031-5183 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T77259 NZ_CP021739:5031-5183 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCGTCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 61 bp

>AT77259 NZ_CP021739:c4979-4919 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin