Detailed information of TA system TA077255

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 94687..95108
• Replicon: plasmid tig00000255
• Accession: NZ_CP021737
• Organism: Escherichia coli strain AR_0150

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: AM397_RS25580
• Protein ID: WP_001302184.1
• Coordinates: 94950..95108 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	94687..94885 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM397_RS25540	90383..91777	+	1395	WP_001037799.1	carbohydrate porin	-
AM397_RS25545	92023..92127	-	105	Protein_98	thiosulfate reductase cytochrome B subunit	-
AM397_RS25555	92184..92877	+	694	Protein_99	IS1 family transposase	-
AM397_RS25560	92893..93516	+	624	WP_000912556.1	potassium channel family protein	-
AM397_RS25565	93601..93852	+	252	Protein_101	iron-containing alcohol dehydrogenase	-
AM397_RS25570	94141..94458	-	318	Protein_102	IS110 family transposase	-
AM397_RS25575	94613..94718	+	106	Protein_103	plasmid SOS inhibition protein A	-
-	94687..94885	+	199	NuclAT_0	-	Antitoxin
-	94687..94885	+	199	NuclAT_0	-	Antitoxin
-	94687..94885	+	199	NuclAT_0	-	Antitoxin
-	94687..94885	+	199	NuclAT_0	-	Antitoxin
AM397_RS25580	94950..95108	+	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
AM397_RS26990	95776..95982	+	207	WP_000275854.1	hypothetical protein	-
AM397_RS25595	96007..96294	+	288	WP_000107242.1	hypothetical protein	-
AM397_RS25600	96413..97234	+	822	WP_001234465.1	DUF945 domain-containing protein	-
AM397_RS25605	97530..98102	-	573	WP_000949660.1	transglycosylase SLT domain-containing protein	-
AM397_RS25610	98472..98855	+	384	WP_001063020.1	relaxosome protein TraM	-
AM397_RS25615	99046..99693	+	648	WP_000332520.1	conjugal transfer transcriptional regulator TraJ	-
AM397_RS25620	99829..100044	+	216	WP_001352842.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / dfrA17 / aadA5 / qacE / sul1 / mph(A)	-	1..117703	117703

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T77255 WP_001302184.1 NZ_CP021737:94950-95108 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T77255 NZ_CP021737:94950-95108 [Escherichia coli]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 199 bp

>AT77255 NZ_CP021737:94687-94885 [Escherichia coli]
TCACACAGATTACCCGTAAACAGTCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin