Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 4836642..4837144 Replicon chromosome
Accession NZ_CP021736
Organism Escherichia coli strain AR_0150

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag AM397_RS24845 Protein ID WP_000767829.1
Coordinates 4836642..4836896 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag AM397_RS24850 Protein ID WP_001259255.1
Coordinates 4836893..4837144 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM397_RS24820 4831657..4831884 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
AM397_RS24825 4831898..4832956 - 1059 WP_000492333.1 transport protein YeeE -
AM397_RS24830 4833135..4834493 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
AM397_RS26740 4834483..4834545 - 63 WP_010723108.1 membrane protein YoeI -
AM397_RS24835 4834760..4835689 - 930 WP_000803351.1 LysR family transcriptional regulator -
AM397_RS24840 4835735..4836559 - 825 WP_000754737.1 SDR family oxidoreductase -
AM397_RS24845 4836642..4836896 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
AM397_RS24850 4836893..4837144 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
AM397_RS24855 4837427..4837477 + 51 WP_001364200.1 his operon leader peptide -
AM397_RS24865 4837623..4838522 + 900 WP_000131792.1 ATP phosphoribosyltransferase -
AM397_RS24870 4838528..4839832 + 1305 WP_001367667.1 histidinol dehydrogenase -
AM397_RS24875 4839829..4840899 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
AM397_RS24880 4840899..4841966 + 1068 WP_000080141.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T77251 WP_000767829.1 NZ_CP021736:c4836896-4836642 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T77251 NZ_CP021736:c4836896-4836642 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT77251 WP_001259255.1 NZ_CP021736:c4837144-4836893 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT77251 NZ_CP021736:c4837144-4836893 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References