Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 2914035..2914729 Replicon chromosome
Accession NZ_CP021736
Organism Escherichia coli strain AR_0150

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag AM397_RS14695 Protein ID WP_001263489.1
Coordinates 2914331..2914729 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag AM397_RS14690 Protein ID WP_000554758.1
Coordinates 2914035..2914328 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM397_RS14670 2909667..2910164 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
AM397_RS14675 2910388..2912100 - 1713 Protein_2672 flagellar biosynthesis protein FlhA -
AM397_RS14680 2912072..2912857 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
AM397_RS14685 2912928..2913983 + 1056 WP_001226164.1 DNA polymerase IV -
AM397_RS14690 2914035..2914328 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
AM397_RS14695 2914331..2914729 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
AM397_RS14700 2914739..2915191 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
AM397_RS14705 2915509..2915715 + 207 Protein_2678 RtcB family protein -
AM397_RS14710 2915711..2916232 + 522 Protein_2679 peptide chain release factor H -
AM397_RS14715 2916289..2917746 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
AM397_RS14720 2918007..2918465 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- 2919061..2919141 + 81 NuclAT_11 - -
- 2919061..2919141 + 81 NuclAT_11 - -
- 2919061..2919141 + 81 NuclAT_11 - -
- 2919061..2919141 + 81 NuclAT_11 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T77238 WP_001263489.1 NZ_CP021736:2914331-2914729 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T77238 NZ_CP021736:2914331-2914729 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT77238 WP_000554758.1 NZ_CP021736:2914035-2914328 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT77238 NZ_CP021736:2914035-2914328 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References