Detailed information of TA system TA077222

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 89753..90007
• Replicon: plasmid unitig_1
• Accession: NZ_CP021733
• Organism: Escherichia coli strain AR_0114

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: AM361_RS25200
• Protein ID: WP_015387399.1
• Coordinates: 89753..89959 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	89946..90007 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM361_RS25165	85000..85911	-	912	WP_000440183.1	carbamate kinase	-
AM361_RS25170	85922..87142	-	1221	WP_000410951.1	arginine deiminase	-
AM361_RS25175	88035..88517	+	483	Protein_116	VENN motif pre-toxin domain-containing protein	-
AM361_RS25180	88463..88909	-	447	Protein_117	incFII family plasmid replication initiator RepA	-
AM361_RS25185	88902..88976	-	75	WP_032336874.1	RepA leader peptide Tap	-
AM361_RS25195	89212..89469	-	258	WP_000083833.1	replication regulatory protein RepA	-
AM361_RS25200	89753..89959	-	207	WP_015387399.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	89946..90007	+	62	NuclAT_1	-	Antitoxin
-	89946..90007	+	62	NuclAT_1	-	Antitoxin
-	89946..90007	+	62	NuclAT_1	-	Antitoxin
-	89946..90007	+	62	NuclAT_1	-	Antitoxin
AM361_RS25210	90146..90328	-	183	WP_000968309.1	hypothetical protein	-
AM361_RS25220	90429..91045	+	617	Protein_122	IS1 family transposase	-
AM361_RS25225	91083..92654	-	1572	WP_023149734.1	IS66 family transposase	-
AM361_RS25230	92674..93021	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
AM361_RS25235	93021..93698	-	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-
AM361_RS26670	93753..93842	+	90	Protein_126	IS1 family transposase	-
AM361_RS25250	94143..94355	-	213	WP_005012601.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / sul1 / qacE / cmlA1 / ant(3'')-Ia / ant(2'')-Ia / blaTEM-1B / sul2 / aph(3'')-Ib / aph(6)-Id	-	1..114267	114267

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7720.23 Da
• Isoelectric Point: 9.3127

>T77222 WP_015387399.1 NZ_CP021733:c89959-89753 [Escherichia coli]
MKYLNTTDCSLFLAGRSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T77222 NZ_CP021733:c89959-89753 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGGGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT77222 NZ_CP021733:89946-90007 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin