Detailed information of TA system TA077215

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 15553..15979
• Replicon: plasmid unitig_1
• Accession: NZ_CP021733
• Organism: Escherichia coli strain AR_0114

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: AM361_RS24620
• Protein ID: WP_001312861.1
• Coordinates: 15553..15711 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	15755..15979 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM361_RS24590	10927..11616	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
AM361_RS24595	11803..12186	-	384	WP_001151524.1	relaxosome protein TraM	-
AM361_RS24600	12507..13109	+	603	WP_013362798.1	transglycosylase SLT domain-containing protein	-
AM361_RS24605	13406..14227	-	822	WP_001234426.1	DUF945 domain-containing protein	-
AM361_RS24610	14345..14632	-	288	WP_000107535.1	hypothetical protein	-
AM361_RS24620	15553..15711	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	15755..15979	-	225	NuclAT_0	-	Antitoxin
-	15755..15979	-	225	NuclAT_0	-	Antitoxin
-	15755..15979	-	225	NuclAT_0	-	Antitoxin
-	15755..15979	-	225	NuclAT_0	-	Antitoxin
AM361_RS26460	15791..15979	+	189	WP_001299721.1	hypothetical protein	-
AM361_RS24625	15991..16710	-	720	WP_001276238.1	plasmid SOS inhibition protein A	-
AM361_RS24630	16707..17141	-	435	WP_000845937.1	conjugation system SOS inhibitor PsiB	-
AM361_RS24635	17196..17393	-	198	Protein_25	hypothetical protein	-
AM361_RS24640	17421..17654	-	234	WP_000005990.1	DUF905 family protein	-
AM361_RS24645	17722..18261	-	540	WP_000290840.1	single-stranded DNA-binding protein	-
AM361_RS26650	18287..18493	-	207	WP_000275856.1	hypothetical protein	-
AM361_RS24665	18903..19115	-	213	WP_001348622.1	hypothetical protein	-
AM361_RS24670	19141..19706	-	566	Protein_30	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / sul1 / qacE / cmlA1 / ant(3'')-Ia / ant(2'')-Ia / blaTEM-1B / sul2 / aph(3'')-Ib / aph(6)-Id	-	1..114267	114267

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T77215 WP_001312861.1 NZ_CP021733:c15711-15553 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T77215 NZ_CP021733:c15711-15553 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT77215 NZ_CP021733:c15979-15755 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin