Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 4450115..4450617 Replicon chromosome
Accession NZ_CP021732
Organism Escherichia coli strain AR_0114

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag AM361_RS22710 Protein ID WP_000767829.1
Coordinates 4450363..4450617 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag AM361_RS22705 Protein ID WP_001259255.1
Coordinates 4450115..4450366 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM361_RS22675 4445293..4446360 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
AM361_RS22680 4446360..4447430 - 1071 WP_000108939.1 histidinol-phosphate transaminase -
AM361_RS22685 4447427..4448731 - 1305 WP_001614652.1 histidinol dehydrogenase -
AM361_RS22690 4448737..4449636 - 900 WP_000131777.1 ATP phosphoribosyltransferase -
AM361_RS22700 4449782..4449832 - 51 WP_001364200.1 his operon leader peptide -
AM361_RS22705 4450115..4450366 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
AM361_RS22710 4450363..4450617 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
AM361_RS22715 4450700..4451524 + 825 WP_000754749.1 SDR family oxidoreductase -
AM361_RS22720 4451570..4452499 + 930 WP_000803351.1 LysR family transcriptional regulator -
AM361_RS26425 4452714..4452776 + 63 WP_010723108.1 membrane protein YoeI -
AM361_RS22725 4452766..4454124 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
AM361_RS22730 4454303..4455361 + 1059 WP_042046654.1 transport protein YeeE -
AM361_RS22735 4455375..4455602 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T77205 WP_000767829.1 NZ_CP021732:4450363-4450617 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T77205 NZ_CP021732:4450363-4450617 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT77205 WP_001259255.1 NZ_CP021732:4450115-4450366 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT77205 NZ_CP021732:4450115-4450366 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References