Detailed information of TA system TA077180

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 130842..131111
• Replicon: plasmid pCombat11I9-2
• Accession: NZ_CP021728
• Organism: Escherichia coli strain Combat11I9

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: CDC27_RS27625
• Protein ID: WP_001312861.1
• Coordinates: 130953..131111 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	130842..130907 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDC27_RS27570	125903..126466	+	564	WP_001311069.1	class I SAM-dependent methyltransferase	-
CDC27_RS27580	126618..126866	+	249	WP_002433376.1	hypothetical protein	-
CDC27_RS27600	127339..127866	+	528	WP_000290793.1	single-stranded DNA-binding protein	-
CDC27_RS27605	127922..128155	+	234	WP_000006004.1	DUF905 domain-containing protein	-
CDC27_RS27610	128214..129468	+	1255	Protein_137	ParB/RepB/Spo0J family partition protein	-
CDC27_RS27615	129523..129957	+	435	WP_000845901.1	conjugation system SOS inhibitor PsiB	-
CDC27_RS27620	129954..130673	+	720	WP_001276234.1	plasmid SOS inhibition protein A	-
CDC27_RS29105	130685..130873	-	189	WP_001299721.1	hypothetical protein	-
-	130685..130909	+	225	NuclAT_0	-	-
-	130685..130909	+	225	NuclAT_0	-	-
-	130685..130909	+	225	NuclAT_0	-	-
-	130685..130909	+	225	NuclAT_0	-	-
-	130842..130907	+	66	-	-	Antitoxin
CDC27_RS27625	130953..131111	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
CDC27_RS29310	131802..132008	+	207	WP_000547939.1	hypothetical protein	-
CDC27_RS27645	132033..132320	+	288	WP_000107535.1	hypothetical protein	-
CDC27_RS27650	132441..133262	+	822	WP_001234469.1	DUF945 domain-containing protein	-
CDC27_RS27655	133559..134161	-	603	WP_000243709.1	transglycosylase SLT domain-containing protein	-
CDC27_RS27660	134482..134865	+	384	WP_001151524.1	relaxosome protein TraM	-
CDC27_RS27665	135052..135741	+	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / fosA3 / oqxB / oqxA / sul1 / sul2 / aph(3'')-Ib / aph(6)-Id / sitABCD	iroN / iroE / iroD / iroC / iroB	1..169487	169487

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T77180 WP_001312861.1 NZ_CP021728:130953-131111 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T77180 NZ_CP021728:130953-131111 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT77180 NZ_CP021728:130842-130907 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin