Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2023364..2023866 Replicon chromosome
Accession NZ_CP021726
Organism Escherichia coli strain Combat11I9

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CDC27_RS10505 Protein ID WP_000767829.1
Coordinates 2023364..2023618 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CDC27_RS10510 Protein ID WP_001259255.1
Coordinates 2023615..2023866 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CDC27_RS10475 2018477..2018704 - 228 WP_000985277.1 sulfurtransferase TusA family protein -
CDC27_RS10480 2018718..2019678 - 961 Protein_1904 YeeE/YedE family protein -
CDC27_RS10485 2019857..2021215 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CDC27_RS10490 2021205..2021267 - 63 WP_010723108.1 membrane protein YoeI -
CDC27_RS10495 2021482..2022411 - 930 WP_000803351.1 LysR family transcriptional regulator -
CDC27_RS10500 2022457..2023281 - 825 WP_000754737.1 SDR family oxidoreductase -
CDC27_RS10505 2023364..2023618 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CDC27_RS10510 2023615..2023866 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CDC27_RS10515 2024149..2024199 + 51 WP_001364200.1 his operon leader peptide -
CDC27_RS10525 2024345..2025244 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
CDC27_RS10530 2025250..2026554 + 1305 WP_064186579.1 histidinol dehydrogenase -
CDC27_RS10535 2026551..2027621 + 1071 WP_000108953.1 histidinol-phosphate transaminase -
CDC27_RS10540 2027621..2028688 + 1068 WP_021541051.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T77167 WP_000767829.1 NZ_CP021726:c2023618-2023364 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T77167 NZ_CP021726:c2023618-2023364 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT77167 WP_001259255.1 NZ_CP021726:c2023866-2023615 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT77167 NZ_CP021726:c2023866-2023615 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCTGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References