Detailed information of TA system TA077153

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 1232114..1232485
• Replicon: chromosome
• Accession: NZ_CP021726
• Organism: Escherichia coli strain Combat11I9

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: CDC27_RS06295
• Protein ID: WP_001317028.1
• Coordinates: 1232291..1232485 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	1232114..1232292 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDC27_RS06270	1228069..1229442	+	1374	WP_021577282.1	ATP-dependent RNA helicase DbpA	-
CDC27_RS06275	1229571..1230506	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
CDC27_RS06280	1230558..1231793	-	1236	WP_000040852.1	site-specific integrase	-
CDC27_RS06285	1231795..1232010	-	216	WP_000079604.1	excisionase XisR	-
CDC27_RS06290	1232089..1232298	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
-	1232114..1232292	+	179	NuclAT_0	-	Antitoxin
-	1232114..1232292	+	179	NuclAT_0	-	Antitoxin
-	1232114..1232292	+	179	NuclAT_0	-	Antitoxin
-	1232114..1232292	+	179	NuclAT_0	-	Antitoxin
CDC27_RS06295	1232291..1232485	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
CDC27_RS06300	1232542..1233351	-	810	WP_000166319.1	recombination protein RecT	-
CDC27_RS06305	1233344..1235944	-	2601	WP_072686724.1	exodeoxyribonuclease VIII	-
CDC27_RS06310	1236046..1236321	-	276	WP_072686725.1	hypothetical protein	-
CDC27_RS06315	1236396..1236566	-	171	WP_001352098.1	conserved protein, Rac prophage	-
CDC27_RS06320	1236566..1236787	-	222	WP_000560225.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1230558..1284491	53933

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T77153 WP_001317028.1 NZ_CP021726:c1232485-1232291 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T77153 NZ_CP021726:c1232485-1232291 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT77153 NZ_CP021726:1232114-1232292 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin