Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2091052..2091554 Replicon chromosome
Accession NZ_CP021722
Organism Escherichia coli strain AR_0128

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag AM375_RS12915 Protein ID WP_000767829.1
Coordinates 2091300..2091554 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag AM375_RS12910 Protein ID WP_001259255.1
Coordinates 2091052..2091303 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM375_RS12880 2086230..2087297 - 1068 WP_000080109.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
AM375_RS12885 2087297..2088367 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
AM375_RS12890 2088364..2089668 - 1305 WP_001345878.1 histidinol dehydrogenase -
AM375_RS12895 2089674..2090573 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
AM375_RS12905 2090719..2090769 - 51 WP_001364200.1 his operon leader peptide -
AM375_RS12910 2091052..2091303 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
AM375_RS12915 2091300..2091554 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
AM375_RS12920 2091637..2092461 + 825 WP_000754737.1 SDR family oxidoreductase -
AM375_RS12925 2092507..2093436 + 930 WP_000803351.1 LysR family transcriptional regulator -
AM375_RS28060 2093651..2093713 + 63 WP_010723108.1 membrane protein YoeI -
AM375_RS12930 2093703..2095061 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
AM375_RS12935 2095240..2096298 + 1059 WP_000492324.1 transport protein YeeE -
AM375_RS12940 2096312..2096539 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T77133 WP_000767829.1 NZ_CP021722:2091300-2091554 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T77133 NZ_CP021722:2091300-2091554 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT77133 WP_001259255.1 NZ_CP021722:2091052-2091303 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT77133 NZ_CP021722:2091052-2091303 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References