Detailed information of TA system TA077127

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 57580..58182
• Replicon: chromosome
• Accession: NZ_CP021722
• Organism: Escherichia coli strain AR_0128

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: AM375_RS02570
• Protein ID: WP_000897305.1
• Coordinates: 57871..58182 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AM375_RS02565
• Protein ID: WP_000356397.1
• Coordinates: 57580..57870 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM375_RS02535	53525..54427	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
AM375_RS02540	54424..55059	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
AM375_RS02545	55056..55985	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
AM375_RS02550	56315..56557	-	243	WP_001087409.1	hypothetical protein	-
AM375_RS02555	56776..56994	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
AM375_RS02565	57580..57870	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
AM375_RS02570	57871..58182	-	312	WP_000897305.1	hypothetical protein	Toxin
AM375_RS02575	58411..59319	+	909	WP_001162704.1	alpha/beta hydrolase	-
AM375_RS02580	59383..60324	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
AM375_RS02585	60369..60806	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
AM375_RS02590	60803..61675	-	873	WP_000920762.1	virulence factor BrkB family protein	-
AM375_RS02595	61669..62268	-	600	WP_001295269.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T77127 WP_000897305.1 NZ_CP021722:c58182-57871 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T77127 NZ_CP021722:c58182-57871 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT77127 WP_000356397.1 NZ_CP021722:c57870-57580 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT77127 NZ_CP021722:c57870-57580 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7