Detailed information of TA system TA077124

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 96606..97317
• Replicon: plasmid tig00000793
• Accession: NZ_CP021720
• Organism: Escherichia coli strain AR_0128

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I2UJZ0
• Locus tag: AM375_RS01405
• Protein ID: WP_000162415.1
• Coordinates: 96606..96908 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AM375_RS01410
• Protein ID: WP_000806445.1
• Coordinates: 96979..97317 (+)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM375_RS01375	91625..92305	-	681	WP_000812238.1	hypothetical protein	-
AM375_RS01380	92289..93194	-	906	WP_080028513.1	recombination-associated protein RdgC	-
AM375_RS01385	93254..93907	-	654	WP_000828872.1	hypothetical protein	-
AM375_RS01390	93904..94884	-	981	WP_000046500.1	hypothetical protein	-
AM375_RS01395	94888..95655	-	768	WP_000203293.1	hypothetical protein	-
AM375_RS01400	95652..96443	-	792	WP_000532307.1	hypothetical protein	-
AM375_RS01405	96606..96908	+	303	WP_000162415.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AM375_RS01410	96979..97317	+	339	WP_000806445.1	HigA family addiction module antidote protein	Antitoxin
AM375_RS01420	97596..98324	+	729	WP_080028515.1	hypothetical protein	-
AM375_RS01425	98358..99551	-	1194	WP_001112629.1	hypothetical protein	-
AM375_RS01430	99544..99873	-	330	WP_000542383.1	hypothetical protein	-
AM375_RS01435	100202..100855	-	654	WP_023908597.1	hypothetical protein	-
AM375_RS01440	101124..102035	+	912	WP_080028516.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaNDM-1	mrkJ / mrkF / htpB	1..216378	216378

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (6-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-69)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11818.56 Da
• Isoelectric Point: 9.8739

>T77124 WP_000162415.1 NZ_CP021720:96606-96908 [Escherichia coli]
MTKKINIKDFRDAWLDDFFEFSTPHRKIPPDIHMTLSRKLDIINAATTCKDLRSPPGNRYEELSGKLNGYSSVRVNKQYR
LIFKWVNGKAEDLYLDPHKY

Nucleotide

• Length: 303 bp

>T77124 NZ_CP021720:96606-96908 [Escherichia coli]
ATGACCAAAAAAATTAACATAAAGGATTTCAGGGATGCGTGGCTTGATGATTTTTTTGAATTTTCAACACCACATAGAAA
GATACCTCCTGATATTCATATGACATTGTCACGGAAGTTGGACATTATCAATGCCGCAACTACCTGTAAGGATTTAAGAT
CACCACCAGGTAATCGGTATGAGGAACTGTCAGGGAAGCTAAATGGCTATTCATCAGTAAGGGTGAATAAGCAATATAGG
TTAATTTTTAAGTGGGTTAACGGAAAGGCCGAGGACTTGTATCTCGACCCTCACAAATACTAA

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 13049.98 Da
• Isoelectric Point: 6.4754

>AT77124 WP_000806445.1 NZ_CP021720:96979-97317 [Escherichia coli]
MKQATRKPTTVGDILLYEYLEPLELKINELAEILHVHRNTVSALVNNNRKLTMDMAYRLAKAFDTSVDFWINLQTAVDLW
EVENDMRVQEELSRINTAEKFISQRNLNKKAA

Nucleotide

• Length: 339 bp

>AT77124 NZ_CP021720:96979-97317 [Escherichia coli]
ATGAAACAGGCAACCAGAAAACCGACGACCGTAGGTGATATCCTGCTGTACGAATACCTGGAGCCGTTAGAGCTGAAGAT
CAACGAGTTAGCAGAAATACTTCATGTTCATCGTAACACCGTAAGTGCTCTTGTTAATAACAATCGTAAGCTAACGATGG
ATATGGCATATCGCCTGGCAAAAGCATTCGATACTTCTGTAGATTTTTGGATTAATCTTCAGACCGCAGTAGATCTGTGG
GAAGTCGAAAATGATATGCGCGTTCAGGAAGAGTTAAGTCGTATCAATACTGCTGAAAAATTTATTTCTCAGCGGAACCT
GAATAAAAAAGCAGCCTGA

Structures

Toxin

• AlphaFold DB: A0A631VBQ3