Detailed information of TA system TA077099

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 63121..63391
• Replicon: plasmid tig00000856
• Accession: NZ_CP021711
• Organism: Klebsiella pneumoniae strain AR_0143

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: AM390_RS29800
• Protein ID: WP_001312861.1
• Coordinates: 63233..63391 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	63121..63184 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM390_RS29770	58887..59414	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
AM390_RS29775	59472..59705	+	234	WP_000006003.1	DUF905 family protein	-
AM390_RS29780	59766..61734	+	1969	Protein_66	ParB/RepB/Spo0J family partition protein	-
AM390_RS29785	61803..62237	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
AM390_RS29790	62234..62953	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	62965..63189	+	225	NuclAT_0	-	-
-	62965..63189	+	225	NuclAT_0	-	-
-	62965..63189	+	225	NuclAT_0	-	-
-	62965..63189	+	225	NuclAT_0	-	-
-	63121..63184	-	64	-	-	Antitoxin
AM390_RS29800	63233..63391	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
AM390_RS29805	63749..64174	+	426	WP_000422741.1	IS66 family insertion sequence hypothetical protein	-
AM390_RS29810	64171..64521	+	351	WP_000624722.1	IS66 family insertion sequence element accessory protein TnpB	-
AM390_RS29815	64552..66165	+	1614	WP_000080200.1	IS66-like element ISEc23 family transposase	-
AM390_RS30730	66250..66454	-	205	Protein_73	pilus protein	-
AM390_RS29835	66846..67142	+	297	WP_001272251.1	hypothetical protein	-
AM390_RS29840	67253..68074	+	822	WP_001234445.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-15 / blaTEM-1B	-	1..78638	78638

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T77099 WP_001312861.1 NZ_CP021711:63233-63391 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T77099 NZ_CP021711:63233-63391 [Klebsiella pneumoniae]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT77099 NZ_CP021711:c63184-63121 [Klebsiella pneumoniae]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin