Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 2761958..2762555 Replicon chromosome
Accession NZ_CP021708
Organism Klebsiella pneumoniae strain AR_0143

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag AM390_RS13780 Protein ID WP_004142563.1
Coordinates 2762238..2762555 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag AM390_RS13775 Protein ID WP_004142561.1
Coordinates 2761958..2762245 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM390_RS13745 2758038..2758286 + 249 WP_002893037.1 DUF1158 domain-containing protein -
AM390_RS13750 2758304..2758645 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
AM390_RS13755 2758676..2759791 - 1116 WP_020316605.1 MBL fold metallo-hydrolase -
AM390_RS13760 2759971..2760552 + 582 WP_043906838.1 TetR/AcrR family transcriptional regulator -
AM390_RS13765 2760552..2760920 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
AM390_RS13770 2761040..2761690 + 651 Protein_2646 oxygen-insensitive NAD(P)H nitroreductase -
AM390_RS13775 2761958..2762245 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
AM390_RS13780 2762238..2762555 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AM390_RS13785 2762740..2763783 - 1044 WP_004178895.1 DUF2157 domain-containing protein -
AM390_RS13795 2764450..2765316 - 867 WP_004178894.1 helix-turn-helix transcriptional regulator -
AM390_RS13800 2765425..2766852 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T77089 WP_004142563.1 NZ_CP021708:c2762555-2762238 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T77089 NZ_CP021708:c2762555-2762238 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT77089 WP_004142561.1 NZ_CP021708:c2762245-2761958 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT77089 NZ_CP021708:c2762245-2761958 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCAACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References