Detailed information of TA system TA077074

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 150234..150961
• Replicon: plasmid tig00000161
• Accession: NZ_CP021697
• Organism: Klebsiella pneumoniae strain AR_0158

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: AM404_RS26655
• Protein ID: WP_011251285.1
• Coordinates: 150650..150961 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AM404_RS26650
• Protein ID: WP_011251286.1
• Coordinates: 150234..150653 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM404_RS26620	145826..147193	+	1368	WP_017900870.1	formimidoylglutamate deiminase	-
AM404_RS29670	147718..148253	+	536	Protein_141	transposase	-
AM404_RS26635	148367..148633	-	267	WP_017900868.1	hypothetical protein	-
AM404_RS26640	148737..149705	+	969	WP_074168518.1	IS5-like element IS903B family transposase	-
AM404_RS26645	149751..150164	-	414	WP_017901337.1	hypothetical protein	-
AM404_RS26650	150234..150653	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
AM404_RS26655	150650..150961	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
AM404_RS26660	151166..151624	-	459	Protein_147	DDE-type integrase/transposase/recombinase	-
AM404_RS26665	151862..152842	-	981	WP_012569499.1	IS5-like element ISKpn26 family transposase	-
AM404_RS26670	152911..153876	-	966	WP_017901242.1	DMT family transporter	-
AM404_RS26675	153873..154691	-	819	WP_017901243.1	amino acid ABC transporter ATP-binding protein	-
AM404_RS26680	154696..155358	-	663	WP_017901244.1	amino acid ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla	1..191266	191266
	-	inside	IScluster/Tn	-	-	148737..152842	4105

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T77074 WP_011251285.1 NZ_CP021697:c150961-150650 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T77074 NZ_CP021697:c150961-150650 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTGAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT77074 WP_011251286.1 NZ_CP021697:c150653-150234 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT77074 NZ_CP021697:c150653-150234 [Klebsiella pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1