Detailed information of TA system TA077072

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 5056563..5057160
• Replicon: chromosome
• Accession: NZ_CP021696
• Organism: Klebsiella pneumoniae strain AR_0158

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9J6S5A1
• Locus tag: AM404_RS25365
• Protein ID: WP_004893639.1
• Coordinates: 5056563..5056880 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: AM404_RS25370
• Protein ID: WP_004142561.1
• Coordinates: 5056873..5057160 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM404_RS25345	5052267..5053694	-	1428	WP_009308097.1	MFS transporter	-
AM404_RS25350	5053803..5054669	+	867	WP_157669363.1	helix-turn-helix transcriptional regulator	-
AM404_RS25360	5055335..5056378	+	1044	WP_009308098.1	DUF2157 domain-containing protein	-
AM404_RS25365	5056563..5056880	+	318	WP_004893639.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AM404_RS25370	5056873..5057160	+	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
AM404_RS25375	5057425..5058078	-	654	WP_004178896.1	oxygen-insensitive NAD(P)H nitroreductase	-
AM404_RS25380	5058198..5058566	-	369	WP_004142557.1	MmcQ/YjbR family DNA-binding protein	-
AM404_RS25385	5058566..5059147	-	582	WP_004176968.1	TetR/AcrR family transcriptional regulator	-
AM404_RS25390	5059327..5060442	+	1116	WP_198360713.1	MBL fold metallo-hydrolase	-
AM404_RS25395	5060473..5060814	+	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
AM404_RS25400	5060832..5061080	-	249	WP_002893037.1	DUF1158 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12100.35 Da
• Isoelectric Point: 11.2767

>T77072 WP_004893639.1 NZ_CP021696:5056563-5056880 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPSIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T77072 NZ_CP021696:5056563-5056880 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGTCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT77072 WP_004142561.1 NZ_CP021696:5056873-5057160 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT77072 NZ_CP021696:5056873-5057160 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1