Detailed information of TA system TA077051

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 146427..147084
• Replicon: plasmid tig00000123
• Accession: NZ_CP021695
• Organism: Proteus mirabilis strain AR_0155

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: AM402_RS21550
• Protein ID: WP_000270043.1
• Coordinates: 146427..146777 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AM402_RS21555
• Protein ID: WP_000124640.1
• Coordinates: 146782..147084 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM402_RS21510	142901..143398	-	498	WP_000062185.1	hypothetical protein	-
AM402_RS21515	143401..143889	-	489	WP_001273096.1	DUF1643 domain-containing protein	-
AM402_RS21520	143986..144321	+	336	WP_000683476.1	hypothetical protein	-
AM402_RS21525	144336..144806	-	471	WP_001281821.1	hypothetical protein	-
AM402_RS21530	144799..145170	-	372	WP_000516916.1	hypothetical protein	-
AM402_RS21535	145181..145375	-	195	WP_000343597.1	hypothetical protein	-
AM402_RS21545	145716..146264	-	549	WP_001061574.1	transcriptional regulator	-
AM402_RS21550	146427..146777	+	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AM402_RS21555	146782..147084	+	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
AM402_RS21560	147111..147404	-	294	WP_001239997.1	chromosome segregation protein ParM	-
AM402_RS21565	147492..147764	-	273	WP_001043047.1	HU family DNA-binding protein	-
AM402_RS21570	147822..148367	-	546	WP_032413569.1	thermonuclease family protein	-
AM402_RS21580	148491..149471	-	981	WP_070531634.1	IS5-like element ISKpn26 family transposase	-
AM402_RS21590	149780..150637	-	858	WP_001167032.1	hypothetical protein	-
AM402_RS21595	150624..150854	-	231	WP_000972663.1	hypothetical protein	-
AM402_RS21600	150854..151372	-	519	WP_000210758.1	nitrite reductase	-
AM402_RS21605	151369..151815	-	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul2 / floR / aph(3')-Ia / blaKPC-6 / sul1 / qacE / aadA5	-	1..214441	214441
	-	flank	IS/Tn	-	-	148491..149471	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T77051 WP_000270043.1 NZ_CP021695:146427-146777 [Proteus mirabilis]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T77051 NZ_CP021695:146427-146777 [Proteus mirabilis]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT77051 WP_000124640.1 NZ_CP021695:146782-147084 [Proteus mirabilis]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT77051 NZ_CP021695:146782-147084 [Proteus mirabilis]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9