Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4324633..4325327 Replicon chromosome
Accession NZ_CP021691
Organism Escherichia coli strain AR_0151

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag AM398_RS22260 Protein ID WP_001263489.1
Coordinates 4324633..4325031 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag AM398_RS22265 Protein ID WP_000554758.1
Coordinates 4325034..4325327 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- 4320221..4320301 - 81 NuclAT_11 - -
- 4320221..4320301 - 81 NuclAT_11 - -
- 4320221..4320301 - 81 NuclAT_11 - -
- 4320221..4320301 - 81 NuclAT_11 - -
AM398_RS22230 4320897..4321355 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
AM398_RS22235 4321616..4323073 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
AM398_RS22240 4323130..4323651 - 522 Protein_4018 peptide chain release factor H -
AM398_RS22245 4323647..4323853 - 207 Protein_4019 RtcB family protein -
AM398_RS22255 4324171..4324623 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
AM398_RS22260 4324633..4325031 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
AM398_RS22265 4325034..4325327 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
AM398_RS22270 4325379..4326434 - 1056 WP_001226164.1 DNA polymerase IV -
AM398_RS22275 4326505..4327290 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
AM398_RS22280 4327262..4328974 + 1713 Protein_4025 flagellar biosynthesis protein FlhA -
AM398_RS22285 4329198..4329695 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T77039 WP_001263489.1 NZ_CP021691:c4325031-4324633 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T77039 NZ_CP021691:c4325031-4324633 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT77039 WP_000554758.1 NZ_CP021691:c4325327-4325034 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT77039 NZ_CP021691:c4325327-4325034 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References