Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2403418..2403920 Replicon chromosome
Accession NZ_CP021691
Organism Escherichia coli strain AR_0151

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag AM398_RS12125 Protein ID WP_000767829.1
Coordinates 2403666..2403920 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag AM398_RS12120 Protein ID WP_001259255.1
Coordinates 2403418..2403669 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM398_RS12090 2398596..2399663 - 1068 WP_000080141.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
AM398_RS12095 2399663..2400733 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
AM398_RS12100 2400730..2402034 - 1305 WP_001367667.1 histidinol dehydrogenase -
AM398_RS12105 2402040..2402939 - 900 WP_000131792.1 ATP phosphoribosyltransferase -
AM398_RS12115 2403085..2403135 - 51 WP_001364200.1 his operon leader peptide -
AM398_RS12120 2403418..2403669 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
AM398_RS12125 2403666..2403920 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
AM398_RS12130 2404003..2404827 + 825 WP_000754737.1 SDR family oxidoreductase -
AM398_RS12135 2404873..2405802 + 930 WP_000803351.1 LysR family transcriptional regulator -
AM398_RS25750 2406017..2406079 + 63 WP_010723108.1 membrane protein YoeI -
AM398_RS12140 2406069..2407427 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
AM398_RS12145 2407606..2408664 + 1059 WP_000492333.1 transport protein YeeE -
AM398_RS12150 2408678..2408905 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T77026 WP_000767829.1 NZ_CP021691:2403666-2403920 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T77026 NZ_CP021691:2403666-2403920 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT77026 WP_001259255.1 NZ_CP021691:2403418-2403669 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT77026 NZ_CP021691:2403418-2403669 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References