Detailed information of TA system TA077009

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 10999..11241
• Replicon: plasmid tig00007555j7554
• Accession: NZ_CP021690
• Organism: Escherichia coli strain AR_0058

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: AM437_RS25730
• Protein ID: WP_001312861.1
• Coordinates: 10999..11157 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	11201..11241 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM437_RS25690	6111..6338	-	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-
AM437_RS25695	6426..7103	-	678	WP_001348626.1	PAS domain-containing protein	-
AM437_RS25700	7237..7620	-	384	WP_001151566.1	relaxosome protein TraM	-
AM437_RS25705	7951..8553	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
AM437_RS25710	8850..9671	-	822	WP_001234469.1	DUF945 domain-containing protein	-
AM437_RS25715	9790..10077	-	288	WP_000107535.1	hypothetical protein	-
AM437_RS27300	10102..10308	-	207	WP_000275859.1	hypothetical protein	-
AM437_RS27305	10221..10556	-	336	WP_013023876.1	hypothetical protein	-
AM437_RS25730	10999..11157	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	11201..11241	-	41	NuclAT_1	-	Antitoxin
-	11201..11241	-	41	NuclAT_1	-	Antitoxin
-	11201..11241	-	41	NuclAT_1	-	Antitoxin
-	11201..11241	-	41	NuclAT_1	-	Antitoxin
-	12685..12871	-	187	NuclAT_0	-	-
-	12685..12871	-	187	NuclAT_0	-	-
-	12685..12871	-	187	NuclAT_0	-	-
-	12685..12871	-	187	NuclAT_0	-	-
AM437_RS25745	12883..13602	-	720	WP_001276232.1	plasmid SOS inhibition protein A	-
AM437_RS25750	13599..14033	-	435	WP_000845940.1	conjugation system SOS inhibitor PsiB	-
AM437_RS25755	14088..16046	-	1959	WP_000117179.1	ParB/RepB/Spo0J family partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA17 / aadA5 / qacE / sul1 / mph(A) / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / aac(3)-IId / blaTEM-52B	senB	1..163045	163045

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T77009 WP_001312861.1 NZ_CP021690:c11157-10999 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T77009 NZ_CP021690:c11157-10999 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 41 bp

>AT77009 NZ_CP021690:c11241-11201 [Escherichia coli]
TTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin