Detailed information of TA system TA076985

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 1189562..1190164
• Replicon: chromosome
• Accession: NZ_CP021689
• Organism: Escherichia coli strain AR_0058

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: AM437_RS06080
• Protein ID: WP_000897302.1
• Coordinates: 1189562..1189873 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AM437_RS06085
• Protein ID: WP_000356397.1
• Coordinates: 1189874..1190164 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM437_RS06055	1185476..1186075	+	600	WP_001296610.1	glucose-1-phosphatase	-
AM437_RS06060	1186069..1186941	+	873	WP_000920762.1	virulence factor BrkB family protein	-
AM437_RS06065	1186938..1187375	+	438	WP_000560981.1	D-tyrosyl-tRNA(Tyr) deacylase	-
AM437_RS06070	1187420..1188361	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
AM437_RS06075	1188425..1189333	-	909	WP_001385591.1	alpha/beta hydrolase	-
AM437_RS06080	1189562..1189873	+	312	WP_000897302.1	hypothetical protein	Toxin
AM437_RS06085	1189874..1190164	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
AM437_RS06095	1190523..1190801	+	279	WP_001296612.1	hypothetical protein	-
AM437_RS06105	1191198..1191416	+	219	WP_001251293.1	ribbon-helix-helix domain-containing protein	-
AM437_RS06110	1191601..1192050	-	450	WP_032140890.1	hypothetical protein	-
AM437_RS06115	1192366..1193214	-	849	WP_001038650.1	hypothetical protein	-
AM437_RS06120	1193504..1193746	+	243	WP_001068514.1	ribbon-helix-helix domain-containing protein	-
AM437_RS06125	1193928..1194857	-	930	WP_000027696.1	formate dehydrogenase accessory protein FdhE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T76985 WP_000897302.1 NZ_CP021689:1189562-1189873 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T76985 NZ_CP021689:1189562-1189873 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT76985 WP_000356397.1 NZ_CP021689:1189874-1190164 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT76985 NZ_CP021689:1189874-1190164 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416