Detailed information of TA system TA076956

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 48015..48285
• Replicon: plasmid tig00008015
• Accession: NZ_CP021684
• Organism: Escherichia coli strain AR_0162

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: AM408_RS26050
• Protein ID: WP_001312861.1
• Coordinates: 48127..48285 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	48015..48078 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM408_RS26020	43823..44308	+	486	WP_031311812.1	single-stranded DNA-binding protein	-
AM408_RS26025	44366..44599	+	234	WP_000006003.1	DUF905 family protein	-
AM408_RS26030	44660..46628	+	1969	Protein_52	ParB/RepB/Spo0J family partition protein	-
AM408_RS26035	46697..47131	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
AM408_RS26040	47128..47847	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	47859..48083	+	225	NuclAT_0	-	-
-	47859..48083	+	225	NuclAT_0	-	-
-	47859..48083	+	225	NuclAT_0	-	-
-	47859..48083	+	225	NuclAT_0	-	-
AM408_RS26045	47868..48047	-	180	WP_001309233.1	hypothetical protein	-
-	48015..48078	-	64	-	-	Antitoxin
AM408_RS26050	48127..48285	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
AM408_RS26915	48523..48900	-	378	Protein_57	hypothetical protein	-
AM408_RS26070	49200..49496	+	297	WP_001272251.1	hypothetical protein	-
AM408_RS26075	49607..50428	+	822	WP_001234445.1	DUF945 domain-containing protein	-
AM408_RS26085	50506..52077	-	1572	WP_000381395.1	IS66 family transposase	-
AM408_RS26090	52097..52444	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
AM408_RS26095	52444..53121	-	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	erm(B) / mph(A) / blaTEM-1B	-	1..84929	84929

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T76956 WP_001312861.1 NZ_CP021684:48127-48285 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T76956 NZ_CP021684:48127-48285 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT76956 NZ_CP021684:c48078-48015 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin