Detailed information of TA system TA076951

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3419970..3420664
• Replicon: chromosome
• Accession: NZ_CP021683
• Organism: Escherichia coli strain AR_0162

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: AM408_RS18785
• Protein ID: WP_001263489.1
• Coordinates: 3419970..3420368 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: AM408_RS18790
• Protein ID: WP_000554758.1
• Coordinates: 3420371..3420664 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	3415558..3415638	-	81	NuclAT_13	-	-
-	3415558..3415638	-	81	NuclAT_13	-	-
-	3415558..3415638	-	81	NuclAT_13	-	-
-	3415558..3415638	-	81	NuclAT_13	-	-
AM408_RS18755	3416234..3416692	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
AM408_RS18760	3416953..3418410	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
AM408_RS18765	3418467..3418988	-	522	Protein_3232	peptide chain release factor H	-
AM408_RS18770	3418984..3419190	-	207	Protein_3233	RtcB family protein	-
AM408_RS18780	3419508..3419960	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
AM408_RS18785	3419970..3420368	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
AM408_RS18790	3420371..3420664	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
AM408_RS18795	3420716..3421771	-	1056	WP_001226164.1	DNA polymerase IV	-
AM408_RS18800	3421842..3422627	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
AM408_RS18805	3422599..3424311	+	1713	Protein_3239	flagellar biosynthesis protein FlhA	-
AM408_RS18810	3424535..3425032	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T76951 WP_001263489.1 NZ_CP021683:c3420368-3419970 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T76951 NZ_CP021683:c3420368-3419970 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT76951 WP_000554758.1 NZ_CP021683:c3420664-3420371 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT76951 NZ_CP021683:c3420664-3420371 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3