Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3419970..3420664 Replicon chromosome
Accession NZ_CP021683
Organism Escherichia coli strain AR_0162

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag AM408_RS18785 Protein ID WP_001263489.1
Coordinates 3419970..3420368 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag AM408_RS18790 Protein ID WP_000554758.1
Coordinates 3420371..3420664 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- 3415558..3415638 - 81 NuclAT_13 - -
- 3415558..3415638 - 81 NuclAT_13 - -
- 3415558..3415638 - 81 NuclAT_13 - -
- 3415558..3415638 - 81 NuclAT_13 - -
AM408_RS18755 3416234..3416692 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
AM408_RS18760 3416953..3418410 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
AM408_RS18765 3418467..3418988 - 522 Protein_3232 peptide chain release factor H -
AM408_RS18770 3418984..3419190 - 207 Protein_3233 RtcB family protein -
AM408_RS18780 3419508..3419960 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
AM408_RS18785 3419970..3420368 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
AM408_RS18790 3420371..3420664 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
AM408_RS18795 3420716..3421771 - 1056 WP_001226164.1 DNA polymerase IV -
AM408_RS18800 3421842..3422627 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
AM408_RS18805 3422599..3424311 + 1713 Protein_3239 flagellar biosynthesis protein FlhA -
AM408_RS18810 3424535..3425032 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T76951 WP_001263489.1 NZ_CP021683:c3420368-3419970 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T76951 NZ_CP021683:c3420368-3419970 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT76951 WP_000554758.1 NZ_CP021683:c3420664-3420371 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT76951 NZ_CP021683:c3420664-3420371 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References