Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1524518..1525020 Replicon chromosome
Accession NZ_CP021683
Organism Escherichia coli strain AR_0162

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag AM408_RS08740 Protein ID WP_000767829.1
Coordinates 1524766..1525020 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag AM408_RS08735 Protein ID WP_001259255.1
Coordinates 1524518..1524769 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM408_RS08705 1519696..1520763 - 1068 WP_000080136.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
AM408_RS08710 1520763..1521833 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
AM408_RS08715 1521836..1523134 - 1299 WP_087760826.1 histidinol dehydrogenase -
AM408_RS08720 1523140..1524039 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
AM408_RS08730 1524185..1524235 - 51 WP_001364200.1 his operon leader peptide -
AM408_RS08735 1524518..1524769 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
AM408_RS08740 1524766..1525020 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
AM408_RS08745 1525103..1525927 + 825 WP_000754762.1 SDR family oxidoreductase -
AM408_RS08750 1525973..1526902 + 930 WP_087760827.1 LysR family transcriptional regulator -
AM408_RS26410 1527117..1527179 + 63 WP_010723108.1 membrane protein YoeI -
AM408_RS08755 1527169..1528527 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
AM408_RS08760 1528706..1529764 + 1059 WP_000492324.1 transport protein YeeE -
AM408_RS08765 1529778..1530005 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T76937 WP_000767829.1 NZ_CP021683:1524766-1525020 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T76937 NZ_CP021683:1524766-1525020 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT76937 WP_001259255.1 NZ_CP021683:1524518-1524769 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT76937 NZ_CP021683:1524518-1524769 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References