Detailed information of TA system TA076882

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3839126..3839799
• Replicon: chromosome
• Accession: NZ_CP021645
• Organism: Pseudomonas psychrotolerans strain CS51

Toxin (Protein)

• Gene name: graT
• Uniprot ID: A0A147GTG8
• Locus tag: CCZ28_RS17265
• Protein ID: WP_058760161.1
• Coordinates: 3839521..3839799 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CCZ28_RS17260
• Protein ID: WP_140219978.1
• Coordinates: 3839126..3839428 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCZ28_RS17240	3835225..3836511	-	1287	WP_140219970.1	TRAP transporter large permease	-
CCZ28_RS17245	3836508..3837002	-	495	WP_140219972.1	TRAP transporter small permease subunit	-
CCZ28_RS17250	3837006..3838007	-	1002	WP_140219974.1	TRAP transporter substrate-binding protein	-
CCZ28_RS17255	3838116..3839024	-	909	WP_140219976.1	alpha/beta hydrolase	-
CCZ28_RS17260	3839126..3839428	-	303	WP_140219978.1	HigA family addiction module antidote protein	Antitoxin
CCZ28_RS17265	3839521..3839799	-	279	WP_058760161.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CCZ28_RS17270	3840025..3840360	-	336	WP_140219980.1	hypothetical protein	-
CCZ28_RS17275	3840756..3842219	+	1464	WP_140219982.1	alginate export family protein	-
CCZ28_RS17280	3842230..3843459	+	1230	WP_140219984.1	3-(3-hydroxy-phenyl)propionate transporter MhpT	-
CCZ28_RS17285	3843452..3844696	+	1245	WP_140219986.1	thiolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-83)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10851.24 Da
• Isoelectric Point: 7.2455

>T76882 WP_058760161.1 NZ_CP021645:c3839799-3839521 [Pseudomonas psychrotolerans]
MIRSFRCSDTEELFTTGQNRQWSQIAAVATRKLAMLNEAIELRDLRSPPGNRLEQLERDRKGQHSIRINDQWRICFEWTA
HGPVNVEIVDYH

Nucleotide

• Length: 279 bp

>T76882 NZ_CP021645:c3839799-3839521 [Pseudomonas psychrotolerans]
ATGATCCGCAGCTTTCGCTGTAGCGATACCGAAGAGCTGTTTACGACTGGACAGAACCGGCAGTGGAGCCAGATAGCTGC
CGTGGCGACGCGGAAGCTGGCGATGTTGAACGAGGCCATTGAGCTGCGAGACCTCAGGTCTCCTCCCGGCAACCGCTTGG
AGCAGCTGGAGCGGGATCGTAAAGGCCAGCACAGCATTCGCATAAACGATCAGTGGCGCATCTGTTTCGAGTGGACCGCA
CACGGGCCGGTCAATGTAGAGATCGTCGACTATCACTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 10772.55 Da
• Isoelectric Point: 8.6017

>AT76882 WP_140219978.1 NZ_CP021645:c3839428-3839126 [Pseudomonas psychrotolerans]
MLTNGMRPVHPGEILREEYLVPMGISASALARALGVSAPTVNDVVLERRGVTADMALRLAAAFDTTPGFWVGLQATYELR
KAEIAKGEQIRAQVKPLQVA

Nucleotide

• Length: 303 bp

>AT76882 NZ_CP021645:c3839428-3839126 [Pseudomonas psychrotolerans]
ATGCTGACCAACGGAATGCGCCCGGTTCATCCCGGCGAGATCCTGCGGGAAGAGTACCTGGTACCCATGGGCATAAGCGC
TTCCGCGCTGGCCCGCGCCCTGGGCGTCTCGGCCCCTACCGTAAACGATGTCGTTCTGGAGCGTCGCGGAGTGACGGCCG
ATATGGCGCTACGCCTCGCCGCTGCGTTCGATACTACCCCTGGTTTCTGGGTAGGCCTGCAGGCTACCTATGAACTGAGA
AAGGCAGAGATAGCGAAAGGTGAACAGATCCGTGCGCAGGTAAAACCTCTGCAGGTCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A147GTG8