Detailed information of TA system TA076877

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 1061879..1062522
• Replicon: chromosome
• Accession: NZ_CP021644
• Organism: Glaesserella parasuis 29755

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U4SMB1
• Locus tag: B4U42_RS05770
• Protein ID: WP_005714197.1
• Coordinates: 1061879..1062223 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: B4U42_RS05775
• Protein ID: WP_005714195.1
• Coordinates: 1062226..1062522 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B4U42_RS05755	1057592..1058220	-	629	Protein_1065	transposase	-
B4U42_RS05760	1058483..1059574	-	1092	WP_005714201.1	murein transglycosylase A	-
B4U42_RS05765	1059685..1061664	-	1980	WP_005714199.1	exoribonuclease II	-
B4U42_RS05770	1061879..1062223	+	345	WP_005714197.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
B4U42_RS05775	1062226..1062522	+	297	WP_005714195.1	helix-turn-helix domain-containing protein	Antitoxin
B4U42_RS05780	1062579..1063541	+	963	Protein_1070	WYL domain-containing protein	-
B4U42_RS05785	1063617..1064390	+	774	WP_005714194.1	PD-(D/E)XK nuclease family protein	-
B4U42_RS05790	1064407..1065054	+	648	WP_005714192.1	hypothetical protein	-
B4U42_RS05795	1065333..1066049	+	717	WP_020996973.1	hypothetical protein	-
B4U42_RS05800	1066094..1067302	+	1209	WP_112065011.1	IS256 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1066094..1067302	1208

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (52-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-76)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13263.22 Da
• Isoelectric Point: 5.9233

>T76877 WP_005714197.1 NZ_CP021644:1061879-1062223 [Glaesserella parasuis 29755]
METEEYLTFIETKVFEEDRKALMSDDEYQKFQAYLLESHELGDFIQNTGGCQKIRWKLESNNKGKSGGVRVIYYVVTKQG
KLLLMMMYAKSKQDNMSDKQKAMLKAVVSQLSEE

Nucleotide

• Length: 345 bp

>T76877 NZ_CP021644:1061879-1062223 [Glaesserella parasuis 29755]
ATGGAAACAGAAGAATATCTTACTTTTATTGAAACCAAAGTTTTTGAAGAAGACCGAAAAGCATTAATGTCTGATGATGA
ATATCAGAAATTTCAGGCTTATTTATTAGAATCTCACGAATTAGGCGATTTTATTCAGAATACTGGTGGTTGCCAAAAAA
TTCGCTGGAAACTAGAAAGTAATAACAAGGGCAAAAGTGGTGGTGTAAGGGTGATTTACTATGTTGTAACCAAACAGGGA
AAATTATTATTAATGATGATGTATGCTAAAAGCAAACAAGACAATATGAGTGATAAACAAAAAGCAATGTTAAAAGCGGT
TGTATCCCAATTAAGTGAGGAGTAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11166.09 Da
• Isoelectric Point: 8.8246

>AT76877 WP_005714195.1 NZ_CP021644:1062226-1062522 [Glaesserella parasuis 29755]
MEKELFERLMESAREMVAIEKGEIEVPPHRIHIHKIPNVKAIRTQHQMKQAEFASLLGVSQNLVQSWEIGRRAPNGAARK
LLAIIEQQPELIPVLQGL

Nucleotide

• Length: 297 bp

>AT76877 NZ_CP021644:1062226-1062522 [Glaesserella parasuis 29755]
ATGGAAAAAGAACTTTTTGAACGTCTAATGGAAAGTGCGAGAGAAATGGTCGCCATTGAAAAAGGCGAAATAGAAGTACC
ACCGCATCGTATTCATATCCATAAAATCCCGAATGTCAAAGCGATTAGAACCCAGCACCAGATGAAACAAGCGGAATTTG
CTTCCCTTTTAGGCGTAAGCCAAAATCTTGTGCAGTCGTGGGAAATCGGCAGACGAGCCCCAAATGGCGCTGCACGGAAA
TTGCTTGCGATTATTGAGCAACAGCCAGAACTTATCCCTGTATTACAAGGGCTTTAG

Structures

Toxin

• AlphaFold DB: U4SMB1