Detailed information of TA system TA076849

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 48224..48881
• Replicon: plasmid tig00000137
• Accession: NZ_CP021551
• Organism: Proteus mirabilis strain AR_0159

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: AM405_RS19050
• Protein ID: WP_000270043.1
• Coordinates: 48224..48574 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AM405_RS19055
• Protein ID: WP_000124640.1
• Coordinates: 48579..48881 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM405_RS19010	44698..45195	-	498	WP_000062185.1	hypothetical protein	-
AM405_RS19015	45198..45686	-	489	WP_001273096.1	DUF1643 domain-containing protein	-
AM405_RS19020	45783..46118	+	336	WP_000683477.1	hypothetical protein	-
AM405_RS19025	46133..46603	-	471	WP_001281821.1	hypothetical protein	-
AM405_RS19030	46596..46967	-	372	Protein_57	hypothetical protein	-
AM405_RS19035	46978..47172	-	195	WP_000343597.1	hypothetical protein	-
AM405_RS19045	47513..48061	-	549	WP_001061573.1	hypothetical protein	-
AM405_RS19050	48224..48574	+	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AM405_RS19055	48579..48881	+	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
AM405_RS19060	48908..49201	-	294	WP_001239998.1	hypothetical protein	-
AM405_RS19065	49290..49562	-	273	WP_001043046.1	HU family DNA-binding protein	-
AM405_RS19070	49620..50147	-	528	WP_001210659.1	thermonuclease family protein	-
AM405_RS20010	50150..50455	-	306	WP_171820680.1	hypothetical protein	-
AM405_RS19080	50367..51224	-	858	WP_001167036.1	hypothetical protein	-
AM405_RS19085	51211..51441	-	231	WP_000972665.1	hypothetical protein	-
AM405_RS19090	51441..51959	-	519	WP_000210757.1	nitrite reductase	-
AM405_RS19095	51956..52402	-	447	WP_000919343.1	hypothetical protein	-
AM405_RS19100	52402..52761	-	360	WP_000422769.1	hypothetical protein	-
AM405_RS19105	52819..53247	-	429	WP_000591076.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mph(E) / msr(E) / armA / sul1 / blaNDM-1 / qacE / aadA2 / dfrA12	-	1..161830	161830

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T76849 WP_000270043.1 NZ_CP021551:48224-48574 [Proteus mirabilis]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T76849 NZ_CP021551:48224-48574 [Proteus mirabilis]
ATGTGGGTCATCGAGACAACTGACACCTTTGATGAGTGGTTCGATGCACTGGATGATACCGATAGAGCCAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGTCCCATGCTGTCGAGACCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTGCGGGTCCAAAGCAAAGGAGATCCTATCAGGGCATTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGCGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT76849 WP_000124640.1 NZ_CP021551:48579-48881 [Proteus mirabilis]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT76849 NZ_CP021551:48579-48881 [Proteus mirabilis]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9