Detailed information of TA system TA076801

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 37231..37942
• Replicon: plasmid unitig_3
• Accession: NZ_CP021537
• Organism: Escherichia coli strain AR_0119

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I2UJZ0
• Locus tag: AM366_RS28205
• Protein ID: WP_000162415.1
• Coordinates: 37640..37942 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AM366_RS28200
• Protein ID: WP_000806445.1
• Coordinates: 37231..37569 (-)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM366_RS28170	32601..33209	-	609	WP_032323273.1	hypothetical protein	-
AM366_RS28175	33493..34146	+	654	WP_087758030.1	maturation control protein	-
AM366_RS28180	34475..34804	+	330	WP_000542383.1	hypothetical protein	-
AM366_RS28185	34797..35990	+	1194	WP_087758031.1	phage tail protein	-
AM366_RS28190	36024..36752	-	729	WP_087758032.1	hypothetical protein	-
AM366_RS28195	36815..37174	-	360	WP_044868318.1	transcriptional regulator	-
AM366_RS28200	37231..37569	-	339	WP_000806445.1	HigA family addiction module antidote protein	Antitoxin
AM366_RS28205	37640..37942	-	303	WP_000162415.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AM366_RS28210	38105..38896	+	792	WP_000532307.1	hypothetical protein	-
AM366_RS28215	38893..39660	+	768	WP_000203293.1	hypothetical protein	-
AM366_RS28220	39664..40644	+	981	WP_000046500.1	hypothetical protein	-
AM366_RS28225	40641..41294	+	654	WP_000828873.1	hypothetical protein	-
AM366_RS28230	41354..42259	+	906	WP_001258019.1	recombination-associated protein RdgC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..97474	97474

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (6-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-69)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11818.56 Da
• Isoelectric Point: 9.8739

>T76801 WP_000162415.1 NZ_CP021537:c37942-37640 [Escherichia coli]
MTKKINIKDFRDAWLDDFFEFSTPHRKIPPDIHMTLSRKLDIINAATTCKDLRSPPGNRYEELSGKLNGYSSVRVNKQYR
LIFKWVNGKAEDLYLDPHKY

Nucleotide

• Length: 303 bp

>T76801 NZ_CP021537:c37942-37640 [Escherichia coli]
ATGACCAAAAAAATTAACATAAAGGATTTCAGGGATGCGTGGCTTGATGATTTTTTTGAATTTTCAACACCACATAGAAA
GATACCTCCTGATATTCATATGACATTGTCACGGAAGTTGGACATTATCAATGCCGCAACTACCTGTAAGGATTTAAGAT
CACCACCAGGTAATCGGTATGAGGAACTGTCAGGGAAGCTAAATGGCTATTCATCAGTAAGGGTGAATAAGCAATATAGG
TTAATTTTTAAGTGGGTTAACGGAAAGGCCGAGGACTTGTATCTCGACCCTCACAAATACTAA

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 13049.98 Da
• Isoelectric Point: 6.4754

>AT76801 WP_000806445.1 NZ_CP021537:c37569-37231 [Escherichia coli]
MKQATRKPTTVGDILLYEYLEPLELKINELAEILHVHRNTVSALVNNNRKLTMDMAYRLAKAFDTSVDFWINLQTAVDLW
EVENDMRVQEELSRINTAEKFISQRNLNKKAA

Nucleotide

• Length: 339 bp

>AT76801 NZ_CP021537:c37569-37231 [Escherichia coli]
ATGAAACAGGCAACCAGAAAACCGACGACCGTAGGTGATATCCTGCTGTACGAATACCTGGAGCCGTTAGAGCTGAAGAT
CAACGAGTTAGCAGAAATACTTCATGTTCATCGTAACACCGTAAGCGCTCTTGTTAATAACAATCGTAAGCTAACGATGG
ATATGGCATATCGCCTGGCAAAAGCATTCGATACTTCTGTAGATTTTTGGATTAATCTTCAGACCGCAGTAGATCTGTGG
GAAGTCGAAAATGATATGCGCGTCCAGGAAGAGTTAAGTCGTATCAATACTGCTGAAAAATTTATTTCTCAGCGGAACCT
GAATAAAAAAGCAGCCTGA

Structures

Toxin

• AlphaFold DB: A0A631VBQ3