Detailed information of TA system TA076795

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 185289..185710
• Replicon: plasmid unitig_2
• Accession: NZ_CP021536
• Organism: Escherichia coli strain AR_0119

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: AM366_RS27490
• Protein ID: WP_001302184.1
• Coordinates: 185289..185447 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	185512..185710 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM366_RS27450	180353..180568	-	216	WP_001352842.1	conjugal transfer relaxosome protein TraY	-
AM366_RS27455	180704..181351	-	648	WP_000332520.1	conjugal transfer transcriptional regulator TraJ	-
AM366_RS27460	181542..181925	-	384	WP_001063020.1	relaxosome protein TraM	-
AM366_RS27465	182295..182867	+	573	WP_000949660.1	transglycosylase SLT domain-containing protein	-
AM366_RS27470	183163..183984	-	822	WP_001234465.1	DUF945 domain-containing protein	-
AM366_RS27475	184103..184390	-	288	WP_000107242.1	hypothetical protein	-
AM366_RS29815	184415..184621	-	207	WP_000275854.1	hypothetical protein	-
AM366_RS29820	184623..184916	-	294	WP_175271036.1	hypothetical protein	-
AM366_RS27490	185289..185447	-	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	185512..185710	-	199	NuclAT_0	-	Antitoxin
-	185512..185710	-	199	NuclAT_0	-	Antitoxin
-	185512..185710	-	199	NuclAT_0	-	Antitoxin
-	185512..185710	-	199	NuclAT_0	-	Antitoxin
AM366_RS27495	185679..185784	-	106	Protein_239	plasmid SOS inhibition protein A	-
AM366_RS27500	185939..186256	+	318	Protein_240	IS110 family transposase	-
AM366_RS27505	186545..186796	-	252	Protein_241	iron-containing alcohol dehydrogenase	-
AM366_RS27510	186881..187504	-	624	WP_000912556.1	potassium channel family protein	-
AM366_RS27515	187520..188213	-	694	Protein_243	IS1 family transposase	-
AM366_RS27525	188270..188374	+	105	Protein_244	thiosulfate reductase cytochrome B subunit	-
AM366_RS27530	188620..190014	-	1395	WP_001037799.1	carbohydrate porin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(6')-Ib / qacE / sul1 / blaNDM-1 / rmtC / blaCTX-M-15 / aac(3)-IIa / blaTEM-1B / blaCMY-6	htpB	1..244955	244955
	-	flank	IS/Tn	-	-	187520..187897	377

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T76795 WP_001302184.1 NZ_CP021536:c185447-185289 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T76795 NZ_CP021536:c185447-185289 [Escherichia coli]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 199 bp

>AT76795 NZ_CP021536:c185710-185512 [Escherichia coli]
TCACACAGATTACCCGTAAACAGTCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin