Detailed information of TA system TA076793

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 137878..138132
• Replicon: plasmid unitig_2
• Accession: NZ_CP021536
• Organism: Escherichia coli strain AR_0119

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: AM366_RS27200
• Protein ID: WP_032215358.1
• Coordinates: 137878..138084 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	138071..138132 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM366_RS27150	133235..133650	-	416	Protein_173	IS1 family transposase	-
AM366_RS27160	133899..134300	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
AM366_RS29345	134233..134490	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
AM366_RS27165	134583..135236	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
AM366_RS27180	136177..137034	-	858	WP_029702152.1	incFII family plasmid replication initiator RepA	-
AM366_RS27185	137027..137101	-	75	WP_001365705.1	RepA leader peptide Tap	-
AM366_RS27195	137337..137594	-	258	WP_000083833.1	replication regulatory protein RepA	-
AM366_RS27200	137878..138084	-	207	WP_032215358.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	138071..138132	+	62	NuclAT_1	-	Antitoxin
-	138071..138132	+	62	NuclAT_1	-	Antitoxin
-	138071..138132	+	62	NuclAT_1	-	Antitoxin
-	138071..138132	+	62	NuclAT_1	-	Antitoxin
AM366_RS29810	138271..138537	-	267	WP_087757657.1	hypothetical protein	-
AM366_RS27215	138912..139481	-	570	WP_029702150.1	DUF2726 domain-containing protein	-
AM366_RS27220	139633..140259	-	627	WP_029702149.1	hypothetical protein	-
AM366_RS27225	140671..140880	-	210	WP_039023209.1	hypothetical protein	-
AM366_RS27230	141011..141571	-	561	WP_001567328.1	fertility inhibition protein FinO	-
AM366_RS27235	141626..142372	-	747	WP_052273601.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(6')-Ib / qacE / sul1 / blaNDM-1 / rmtC / blaCTX-M-15 / aac(3)-IIa / blaTEM-1B / blaCMY-6	htpB	1..244955	244955

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7778.27 Da
• Isoelectric Point: 8.8807

>T76793 WP_032215358.1 NZ_CP021536:c138084-137878 [Escherichia coli]
MKYLNTTDCSLFLAERSKFMTKYALIGVLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T76793 NZ_CP021536:c138084-137878 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GGTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT76793 NZ_CP021536:138071-138132 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin