Detailed information of TA system TA076792

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 88227..88884
• Replicon: plasmid unitig_2
• Accession: NZ_CP021536
• Organism: Escherichia coli strain AR_0119

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: AM366_RS26815
• Protein ID: WP_000270043.1
• Coordinates: 88534..88884 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AM366_RS26810
• Protein ID: WP_000124640.1
• Coordinates: 88227..88529 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM366_RS26760	83861..84289	+	429	WP_000591076.1	hypothetical protein	-
AM366_RS26765	84347..84706	+	360	WP_000422769.1	hypothetical protein	-
AM366_RS26770	84706..85152	+	447	WP_000919343.1	hypothetical protein	-
AM366_RS26775	85149..85667	+	519	WP_000210757.1	nitrite reductase	-
AM366_RS26780	85667..85897	+	231	WP_000972664.1	hypothetical protein	-
AM366_RS26785	85884..86741	+	858	WP_001167036.1	hypothetical protein	-
AM366_RS29785	86653..86958	+	306	WP_171820680.1	hypothetical protein	-
AM366_RS26795	86961..87488	+	528	WP_004201083.1	thermonuclease family protein	-
AM366_RS26800	87546..87818	+	273	WP_001043046.1	HU family DNA-binding protein	-
AM366_RS26805	87907..88200	+	294	WP_001239998.1	hypothetical protein	-
AM366_RS26810	88227..88529	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
AM366_RS26815	88534..88884	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AM366_RS26820	89047..89595	+	549	WP_001061573.1	hypothetical protein	-
AM366_RS26830	89936..90130	+	195	WP_000343597.1	hypothetical protein	-
AM366_RS26835	90141..90512	+	372	WP_000516918.1	hypothetical protein	-
AM366_RS26840	90505..90975	+	471	WP_001281821.1	hypothetical protein	-
AM366_RS26845	90990..91325	-	336	WP_000683477.1	hypothetical protein	-
AM366_RS26850	91422..91910	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
AM366_RS26855	91913..92410	+	498	WP_000062185.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(6')-Ib / qacE / sul1 / blaNDM-1 / rmtC / blaCTX-M-15 / aac(3)-IIa / blaTEM-1B / blaCMY-6	htpB	1..244955	244955

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T76792 WP_000270043.1 NZ_CP021536:c88884-88534 [Escherichia coli]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T76792 NZ_CP021536:c88884-88534 [Escherichia coli]
ATGTGGGTCATCGAGACAACTGACACCTTTGATGAGTGGTTCGATGCACTGGATGATACCGATAGAGCCAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGTCCCATGCTGTCGAGACCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTGCGGGTCCAAAGCAAAGGAGATCCTATCAGGGCATTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGCGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT76792 WP_000124640.1 NZ_CP021536:c88529-88227 [Escherichia coli]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT76792 NZ_CP021536:c88529-88227 [Escherichia coli]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9