Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4374039..4374580 Replicon chromosome
Accession NZ_CP021535
Organism Escherichia coli strain AR_0119

Toxin (Protein)


Gene name yafQ Uniprot ID E9TC49
Locus tag AM366_RS22845 Protein ID WP_000615982.1
Coordinates 4374302..4374580 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag AM366_RS22840 Protein ID WP_000729704.1
Coordinates 4374039..4374299 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM366_RS22815 4369075..4370214 + 1140 WP_000785662.1 flagellar type III secretion system protein FlhB -
AM366_RS22820 4370198..4372291 + 2094 WP_001140806.1 flagellar biosynthesis protein FlhA -
AM366_RS22825 4372396..4372674 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
AM366_RS22830 4372667..4373023 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
AM366_RS22835 4373080..4373853 - 774 WP_000543899.1 C40 family peptidase -
AM366_RS22840 4374039..4374299 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
AM366_RS22845 4374302..4374580 + 279 WP_000615982.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
AM366_RS22850 4374736..4375476 + 741 WP_001225679.1 murein L,D-transpeptidase -
AM366_RS22855 4375447..4376214 - 768 WP_000333380.1 class II glutamine amidotransferase -
AM366_RS22860 4376420..4376998 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10816.59 Da        Isoelectric Point: 10.0702

>T76788 WP_000615982.1 NZ_CP021535:4374302-4374580 [Escherichia coli]
MIQRDIEYSGQFSKDVKVAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T76788 NZ_CP021535:4374302-4374580 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAAGTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT76788 WP_000729704.1 NZ_CP021535:4374039-4374299 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT76788 NZ_CP021535:4374039-4374299 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References