Detailed information of TA system TA076783

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 3125323..3125694
• Replicon: chromosome
• Accession: NZ_CP021535
• Organism: Escherichia coli strain AR_0119

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: AM366_RS16210
• Protein ID: WP_001317028.1
• Coordinates: 3125323..3125517 (+)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	3125516..3125694 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM366_RS16185	3121021..3121242	+	222	WP_000560225.1	killing protein KilR	-
AM366_RS16190	3121242..3121412	+	171	WP_001352098.1	conserved protein, Rac prophage	-
AM366_RS16195	3121487..3121762	+	276	WP_000632297.1	protein RacC	-
AM366_RS16200	3121864..3124464	+	2601	WP_000105127.1	exodeoxyribonuclease VIII	-
AM366_RS16205	3124457..3125239	+	783	WP_060615110.1	recombination protein RecT	-
AM366_RS16210	3125323..3125517	+	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
AM366_RS16215	3125510..3125719	+	210	WP_000276809.1	double-strand break reduction protein RcbA	-
-	3125516..3125694	-	179	NuclAT_1	-	Antitoxin
-	3125516..3125694	-	179	NuclAT_1	-	Antitoxin
-	3125516..3125694	-	179	NuclAT_1	-	Antitoxin
-	3125516..3125694	-	179	NuclAT_1	-	Antitoxin
AM366_RS16220	3125798..3126013	+	216	WP_000079604.1	excisionase XisR	-
AM366_RS16225	3126015..3127250	+	1236	WP_000040852.1	site-specific integrase	-
AM366_RS16230	3127302..3128237	+	936	WP_001153728.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
AM366_RS16235	3128366..3129739	-	1374	WP_000123745.1	ATP-dependent RNA helicase DbpA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3099616..3131200	31584

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T76783 WP_001317028.1 NZ_CP021535:3125323-3125517 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T76783 NZ_CP021535:3125323-3125517 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT76783 NZ_CP021535:c3125694-3125516 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin