Detailed information of TA system TA076768

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 1707503..1707772
• Replicon: chromosome
• Accession: NZ_CP021535
• Organism: Escherichia coli strain AR_0119

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: AM366_RS08905
• Protein ID: WP_001312861.1
• Coordinates: 1707614..1707772 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	1707503..1707568 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM366_RS08875	1703213..1703740	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
AM366_RS08880	1703798..1704031	+	234	WP_000006003.1	DUF905 family protein	-
AM366_RS08885	1704092..1706115	+	2024	Protein_1596	ParB/RepB/Spo0J family partition protein	-
AM366_RS08890	1706184..1706618	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
AM366_RS08895	1706615..1707334	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	1707346..1707570	+	225	NuclAT_0	-	-
-	1707346..1707570	+	225	NuclAT_0	-	-
-	1707346..1707570	+	225	NuclAT_0	-	-
-	1707346..1707570	+	225	NuclAT_0	-	-
AM366_RS08900	1707355..1707534	-	180	WP_001309233.1	hypothetical protein	-
-	1707503..1707568	+	66	-	-	Antitoxin
AM366_RS08905	1707614..1707772	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
AM366_RS08910	1708130..1708555	+	426	WP_000422741.1	IS66 family insertion sequence hypothetical protein	-
AM366_RS08915	1708552..1708902	+	351	WP_000624722.1	IS66 family insertion sequence element accessory protein TnpB	-
AM366_RS08920	1708933..1710546	+	1614	WP_000080200.1	IS66-like element ISEc23 family transposase	-
AM366_RS29640	1710631..1710835	-	205	Protein_1604	pilus protein	-
AM366_RS08940	1711227..1711523	+	297	WP_001272251.1	hypothetical protein	-
AM366_RS08945	1711634..1712455	+	822	WP_001234445.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	blaCTX-M-15	algU	1641851..1827083	185232

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T76768 WP_001312861.1 NZ_CP021535:1707614-1707772 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T76768 NZ_CP021535:1707614-1707772 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT76768 NZ_CP021535:1707503-1707568 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin