Detailed information of TA system TA076758

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 36672..36936
• Replicon: plasmid tig00000220
• Accession: NZ_CP021533
• Organism: Escherichia coli strain AR_0149

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: AM396_RS24995
• Protein ID: WP_001387489.1
• Coordinates: 36784..36936 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	36672..36732 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM396_RS24955	32797..33060	+	264	WP_032180564.1	ash family protein	-
AM396_RS24960	32978..33265	+	288	WP_000074855.1	conjugal transfer protein TraA	-
AM396_RS24970	33707..34012	+	306	Protein_41	transcription termination factor NusG	-
AM396_RS24975	34026..34723	-	698	Protein_42	IS1 family transposase	-
AM396_RS24985	34779..35936	-	1158	Protein_43	IS1380-like element ISEc9 family transposase	-
AM396_RS25720	36122..36469	-	348	Protein_44	protein finQ	-
-	36672..36732	-	61	NuclAT_0	-	Antitoxin
-	36672..36732	-	61	NuclAT_0	-	Antitoxin
-	36672..36732	-	61	NuclAT_0	-	Antitoxin
-	36672..36732	-	61	NuclAT_0	-	Antitoxin
AM396_RS24995	36784..36936	+	153	WP_001387489.1	Hok/Gef family protein	Toxin
AM396_RS25000	37008..37259	-	252	WP_001291964.1	hypothetical protein	-
AM396_RS25005	37559..37855	+	297	WP_011264046.1	hypothetical protein	-
AM396_RS25910	37920..38096	-	177	WP_001054897.1	hypothetical protein	-
AM396_RS25010	38488..38697	+	210	WP_000062603.1	HEAT repeat domain-containing protein	-
AM396_RS25020	38922..39619	+	698	Protein_50	IS1 family transposase	-
AM396_RS25025	40072..41217	+	1146	WP_039023136.1	class C extended-spectrum beta-lactamase CMY-42	-
AM396_RS25030	41311..41844	+	534	WP_001221666.1	lipocalin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCMY-42	-	1..48528	48528
	-	inside	IScluster/Tn	blaCMY-42	-	34448..41217	6769

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T76758 WP_001387489.1 NZ_CP021533:36784-36936 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T76758 NZ_CP021533:36784-36936 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCGTCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 61 bp

>AT76758 NZ_CP021533:c36732-36672 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin