Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3241563..3242065 Replicon chromosome
Accession NZ_CP021532
Organism Escherichia coli strain AR_0149

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag AM396_RS16620 Protein ID WP_000767829.1
Coordinates 3241563..3241817 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag AM396_RS16625 Protein ID WP_001259255.1
Coordinates 3241814..3242065 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM396_RS16595 3236578..3236805 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
AM396_RS16600 3236819..3237877 - 1059 WP_000492333.1 transport protein YeeE -
AM396_RS16605 3238056..3239414 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
AM396_RS25635 3239404..3239466 - 63 WP_010723108.1 membrane protein YoeI -
AM396_RS16610 3239681..3240610 - 930 WP_000803351.1 LysR family transcriptional regulator -
AM396_RS16615 3240656..3241480 - 825 WP_000754737.1 SDR family oxidoreductase -
AM396_RS16620 3241563..3241817 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
AM396_RS16625 3241814..3242065 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
AM396_RS16630 3242348..3242398 + 51 WP_001364200.1 his operon leader peptide -
AM396_RS16640 3242544..3243443 + 900 WP_000131792.1 ATP phosphoribosyltransferase -
AM396_RS16645 3243449..3244753 + 1305 WP_001367667.1 histidinol dehydrogenase -
AM396_RS16650 3244750..3245820 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
AM396_RS16655 3245820..3246887 + 1068 WP_000080141.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T76751 WP_000767829.1 NZ_CP021532:c3241817-3241563 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T76751 NZ_CP021532:c3241817-3241563 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT76751 WP_001259255.1 NZ_CP021532:c3242065-3241814 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT76751 NZ_CP021532:c3242065-3241814 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References