Detailed information of TA system TA076703

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-RelB
• Location: 3134..3675
• Replicon: plasmid pKE1446-2
• Accession: NZ_CP021469
• Organism: Komagataeibacter europaeus strain SRCM101446

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: S101446_RS17335
• Protein ID: WP_087609075.1
• Coordinates: 3406..3675 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: S101446_RS17330
• Protein ID: WP_087609074.1
• Coordinates: 3134..3409 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
S101446_RS17325	7..3081	+	3075	WP_087609098.1	Ti-type conjugative transfer relaxase TraA	-
S101446_RS17330	3134..3409	+	276	WP_087609074.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
S101446_RS17335	3406..3675	+	270	WP_087609075.1	Txe/YoeB family addiction module toxin	Toxin
S101446_RS17340	3688..4356	+	669	WP_087609076.1	hypothetical protein	-
S101446_RS17345	4365..4772	-	408	WP_087609077.1	Rrf2 family transcriptional regulator	-
S101446_RS17350	4763..5041	-	279	WP_087609078.1	hypothetical protein	-
S101446_RS17355	5385..5771	-	387	WP_087609079.1	type II toxin-antitoxin system VapC family toxin	-
S101446_RS17360	5768..6013	-	246	WP_087609099.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
S101446_RS17365	6402..7049	+	648	WP_087609080.1	invasion associated locus B family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..37199	37199

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-89)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-75)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10428.80 Da
• Isoelectric Point: 9.0006

>T76703 WP_087609075.1 NZ_CP021469:3406-3675 [Komagataeibacter europaeus]
VKVIFHEDGWEDYLSWFQSDHAVLGKVNALIEDVRRHPFQGLGKPEPLKGQLSGWWSRRITGEHRLVYRVQGRAGADQRI
EVAQCRFHY

Nucleotide

• Length: 270 bp

>T76703 NZ_CP021469:3406-3675 [Komagataeibacter europaeus]
GTGAAGGTCATTTTTCATGAAGATGGCTGGGAGGATTATCTTTCCTGGTTCCAGTCAGATCATGCTGTTCTTGGGAAGGT
TAATGCCCTGATCGAGGATGTAAGGCGGCATCCATTTCAGGGGCTGGGCAAGCCGGAACCACTGAAGGGACAGCTTTCCG
GGTGGTGGTCCCGACGGATTACGGGGGAACATCGTCTGGTCTACCGGGTTCAGGGCCGCGCGGGAGCGGATCAGCGGATC
GAGGTTGCCCAGTGCCGGTTTCATTACTGA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10193.43 Da
• Isoelectric Point: 5.1213

>AT76703 WP_087609074.1 NZ_CP021469:3134-3409 [Komagataeibacter europaeus]
MTCVSYTDLRQNLAHYLDEAVNSRAPIVVTRKTGKGSVVLMSEEEFSGWQETVHLLSSPRNAERLLRSIRDIEHGVATER
DLLEPQGEPSA

Nucleotide

• Length: 276 bp

>AT76703 NZ_CP021469:3134-3409 [Komagataeibacter europaeus]
ATGACCTGTGTTTCCTATACCGACCTGCGCCAGAACCTGGCGCATTATCTGGACGAAGCCGTGAACAGCCGCGCGCCGAT
TGTCGTGACTCGCAAGACCGGGAAAGGCAGTGTCGTGCTGATGTCGGAAGAAGAATTCTCCGGCTGGCAGGAAACCGTGC
ATCTCCTGAGCAGCCCACGCAACGCGGAGAGGCTGTTGCGGAGCATTCGTGACATCGAGCACGGGGTGGCGACCGAGCGT
GATCTTCTGGAGCCGCAGGGTGAGCCGTCGGCGTGA