Detailed information of TA system TA076673

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/MqsA(antitoxin)
• Location: 164839..165491
• Replicon: plasmid pSEPPX05
• Accession: NZ_CP021449
• Organism: Ralstonia solanacearum strain SEPPX05

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0S4U5H4
• Locus tag: RSSE_RS19600
• Protein ID: WP_028861315.1
• Coordinates: 164839..165183 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: RSSE_RS19605
• Protein ID: WP_020425351.1
• Coordinates: 165180..165491 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RSSE_RS19585	160535..161698	-	1164	WP_011003438.1	2-methylcitrate synthase	-
RSSE_RS19590	161750..162646	-	897	WP_087452989.1	methylisocitrate lyase	-
RSSE_RS19595	162839..164788	+	1950	WP_087452413.1	propionate catabolism operon regulatory protein PrpR	-
RSSE_RS19600	164839..165183	+	345	WP_028861315.1	hypothetical protein	Toxin
RSSE_RS19605	165180..165491	+	312	WP_020425351.1	transcriptional regulator	Antitoxin
RSSE_RS19610	165631..166440	-	810	WP_087452990.1	DUF2242 domain-containing protein	-
RSSE_RS19620	167617..168123	+	507	Protein_137	IS5 family transposase	-
RSSE_RS19630	168644..169911	+	1268	Protein_138	group II intron reverse transcriptase/maturase	-
RSSE_RS19635	169962..170432	+	471	Protein_139	IS5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	motA / cheW / cheB / cheY / adeG / acrB / fliI / fliG / fliM / flgG / flgC / cyaB	1..2066085	2066085

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (41-91)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13276.03 Da
• Isoelectric Point: 9.3395

>T76673 WP_028861315.1 NZ_CP021449:164839-165183 [Ralstonia solanacearum]
MDATFIELPPFQRLREHYLSDDEYRALQQELLARPDAGDVIKGTGGLRKLRFSDKRRGKGKRGGLRVIYYHWDGGGQFWM
FVVYDKGEADDLSSDERKVLARLLEQEVKARSER

Nucleotide

• Length: 345 bp

>T76673 NZ_CP021449:164839-165183 [Ralstonia solanacearum]
ATGGATGCTACCTTTATCGAGTTGCCGCCTTTTCAGAGGCTGCGGGAGCACTATCTCTCGGACGACGAGTACCGAGCGCT
CCAACAGGAACTGCTGGCCCGGCCGGACGCCGGCGACGTCATCAAGGGGACAGGTGGCCTACGCAAGCTGCGATTTTCGG
ACAAGCGGCGCGGCAAAGGTAAGCGCGGCGGTCTGCGCGTGATTTACTACCACTGGGATGGAGGCGGCCAATTCTGGATG
TTTGTCGTCTACGACAAGGGCGAGGCGGACGACCTTTCCAGCGACGAGCGCAAAGTGCTGGCACGGCTGCTGGAGCAGGA
AGTCAAGGCAAGGAGTGAGCGATGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11619.65 Da
• Isoelectric Point: 10.7132

>AT76673 WP_020425351.1 NZ_CP021449:165180-165491 [Ralstonia solanacearum]
MKRNLFAELKEGMDALAAEREGKMTLRKVKVRVLQPLDVTAEEIKAVRAAAHASQAVMARRLRVNVRTYQNWEQGTAKPN
TQAAVLIKLVEKHPETLQMLEAL

Nucleotide

• Length: 312 bp

>AT76673 NZ_CP021449:165180-165491 [Ralstonia solanacearum]
ATGAAACGGAACCTGTTTGCTGAGTTGAAGGAGGGGATGGATGCGCTTGCCGCAGAGCGCGAAGGAAAGATGACCCTTCG
CAAGGTGAAGGTCCGCGTGCTCCAGCCGCTTGACGTGACAGCCGAAGAAATCAAGGCCGTTCGCGCAGCGGCACATGCTT
CACAGGCTGTCATGGCACGTCGACTGCGCGTCAATGTGCGCACCTACCAGAACTGGGAACAAGGAACCGCCAAACCGAAC
ACCCAGGCGGCTGTGTTGATCAAGCTCGTCGAGAAGCATCCGGAAACGTTGCAGATGCTGGAAGCGCTTTAA

Structures

Toxin

• AlphaFold DB: A0A0S4U5H4