Detailed information of TA system TA076614

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 9004..9655
• Replicon: plasmid p11
• Accession: NZ_CP021429
• Organism: Acinetobacter pittii strain HUMV-6483

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S3SZA7
• Locus tag: CCK83_RS19980
• Protein ID: WP_000269903.1
• Coordinates: 9299..9655 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CCK83_RS19975
• Protein ID: WP_001140619.1
• Coordinates: 9004..9306 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCK83_RS19930	4293..5501	-	1209	WP_021511398.1	IS256 family transposase	-
CCK83_RS19935	5598..5885	-	288	WP_001258992.1	helix-turn-helix transcriptional regulator	-
CCK83_RS19940	5872..6060	-	189	WP_016653392.1	type II toxin-antitoxin system RelE/ParE family toxin	-
CCK83_RS19945	6337..6657	-	321	WP_016653393.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
CCK83_RS20750	6845..7233	+	389	Protein_11	AAA family ATPase	-
CCK83_RS19955	7161..7706	+	546	Protein_12	IS5 family transposase	-
CCK83_RS19965	8465..8677	+	213	WP_016653394.1	hypothetical protein	-
CCK83_RS19970	8802..9011	-	210	WP_000069471.1	hypothetical protein	-
CCK83_RS19975	9004..9306	-	303	WP_001140619.1	XRE family transcriptional regulator	Antitoxin
CCK83_RS19980	9299..9655	-	357	WP_000269903.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CCK83_RS19985	10190..10717	-	528	WP_002124964.1	plasmid pRiA4b ORF-3 family protein	-
CCK83_RS19995	10897..12222	+	1326	WP_016805832.1	ISNCY family transposase	-
CCK83_RS20970	12422..12517	-	96	Protein_19	DNA-binding protein	-
CCK83_RS20760	12515..12736	-	222	Protein_20	IS3 family transposase	-
CCK83_RS20975	12871..13155	-	285	Protein_21	antibiotic biosynthesis monooxygenase	-
CCK83_RS20010	13176..14174	-	999	WP_000649019.1	zinc-binding alcohol dehydrogenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..112604	112604
	-	inside	IScluster/Tn	-	-	4293..12222	7929

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (13-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13486.45 Da
• Isoelectric Point: 7.1645

>T76614 WP_000269903.1 NZ_CP021429:c9655-9299 [Acinetobacter pittii]
MWTVITTDLFNEWLAQQDQSTQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKGGKKRFYKEMLDIADQQYEIHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T76614 NZ_CP021429:c9655-9299 [Acinetobacter pittii]
ATGTGGACAGTCATTACAACGGATCTGTTTAACGAATGGCTTGCACAGCAAGATCAATCTACACAAGAGAAAGTACTCGC
AGCGTTGGTCGTTCTACAGCAACAGGGACCAAGTTTAGGTCGTCCTTTAGTAGATACCGTCTATGACTCCAAATTTACCA
ATATGAAAGAGCTACGTGTTCAGCATCGAGGTAAACCACTGAGAGCCTTTTTCGCGTTTGATCCTTTACGACAAGCTATT
GTGCTGTGTATCGGTGATAAAGGCGGTAAAAAGCGTTTTTATAAAGAGATGTTAGATATTGCCGATCAGCAATACGAAAT
TCATCTTTCAACTTTAGGAGATCAATCTAATGGCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11206.01 Da
• Isoelectric Point: 6.7282

>AT76614 WP_001140619.1 NZ_CP021429:c9306-9004 [Acinetobacter pittii]
MAKTLQELLANRSPESQARIQQMADELLLETQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT76614 NZ_CP021429:c9306-9004 [Acinetobacter pittii]
ATGGCTAAGACCCTACAAGAATTATTGGCTAATCGCTCTCCAGAGAGTCAAGCTCGTATTCAACAAATGGCGGATGAATT
ATTGTTGGAAACTCAGCTTCACCTTATTCGTGAAGAGTTAGAGATTTCTCAAAAAGAACTGGCTGCAACCCTTGGAATCA
AACAGCCTTCATTGTCAGCAATCGAAAATCGTGGTCATGATCTTAAAATTTCAACGATGAAAAAGTATGTCGAAGCAATG
GGCGGAAAACTTCGTATTGATGTAGAGCTTCCAACAGGAAAACACATAGGATTCAATGTCTAA

Structures

Toxin

• AlphaFold DB: A0A836MG83