Detailed information of TA system TA076613

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 5598..6060
• Replicon: plasmid p11
• Accession: NZ_CP021429
• Organism: Acinetobacter pittii strain HUMV-6483

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S3T2E4
• Locus tag: CCK83_RS19940
• Protein ID: WP_016653392.1
• Coordinates: 5872..6060 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CCK83_RS19935
• Protein ID: WP_001258992.1
• Coordinates: 5598..5885 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCK83_RS19900	879..1115	+	237	WP_000152660.1	hypothetical protein	-
CCK83_RS19905	1776..2231	+	456	WP_001136764.1	hypothetical protein	-
CCK83_RS19910	2292..2759	+	468	WP_005071563.1	hypothetical protein	-
CCK83_RS19915	2728..3369	+	642	WP_021511117.1	hypothetical protein	-
CCK83_RS19920	3429..3970	+	542	Protein_5	DUF2280 domain-containing protein	-
CCK83_RS19925	3954..4253	+	300	Protein_6	terminase	-
CCK83_RS19930	4293..5501	-	1209	WP_021511398.1	IS256 family transposase	-
CCK83_RS19935	5598..5885	-	288	WP_001258992.1	helix-turn-helix transcriptional regulator	Antitoxin
CCK83_RS19940	5872..6060	-	189	WP_016653392.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CCK83_RS19945	6337..6657	-	321	WP_016653393.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
CCK83_RS20750	6845..7233	+	389	Protein_11	AAA family ATPase	-
CCK83_RS19955	7161..7706	+	546	Protein_12	IS5 family transposase	-
CCK83_RS19965	8465..8677	+	213	WP_016653394.1	hypothetical protein	-
CCK83_RS19970	8802..9011	-	210	WP_000069471.1	hypothetical protein	-
CCK83_RS19975	9004..9306	-	303	WP_001140619.1	XRE family transcriptional regulator	-
CCK83_RS19980	9299..9655	-	357	WP_000269903.1	type II toxin-antitoxin system RelE/ParE family toxin	-
CCK83_RS19985	10190..10717	-	528	WP_002124964.1	plasmid pRiA4b ORF-3 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..112604	112604
	-	inside	IScluster/Tn	-	-	4293..12222	7929

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (2-55)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-90)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7183.65 Da
• Isoelectric Point: 10.7295

>T76613 WP_016653392.1 NZ_CP021429:c6060-5872 [Acinetobacter pittii]
MSKGLFELRLKSQECIARVMYCTLVGKRIVMLHSFVKKTQKTPKQDLNLALDRMKEVKNANT

Nucleotide

• Length: 189 bp

>T76613 NZ_CP021429:c6060-5872 [Acinetobacter pittii]
ATGAGTAAAGGACTATTTGAGTTGCGCTTAAAATCTCAAGAATGTATTGCTCGTGTTATGTATTGCACACTAGTCGGTAA
ACGCATTGTAATGCTCCATAGTTTTGTTAAGAAAACCCAAAAAACACCTAAGCAAGATCTAAACCTAGCACTTGACCGCA
TGAAAGAGGTGAAAAATGCGAACACATGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10563.32 Da
• Isoelectric Point: 9.7308

>AT76613 WP_001258992.1 NZ_CP021429:c5885-5598 [Acinetobacter pittii]
MRTHEEMKALALSRSAVRTEYERIEREEMPLLDMVLTALREAGLSQAQIAERMGTNVPAVSRLEKALITGKPSPSIATLQ
KYAAAIGKHVEVRFV

Nucleotide

• Length: 288 bp

>AT76613 NZ_CP021429:c5885-5598 [Acinetobacter pittii]
ATGCGAACACATGAAGAAATGAAAGCACTTGCATTATCTCGTTCAGCAGTGCGTACCGAATACGAACGAATTGAACGTGA
AGAAATGCCCTTGCTCGATATGGTACTTACTGCACTTCGAGAGGCAGGTCTATCCCAAGCGCAAATCGCGGAACGCATGG
GAACAAATGTACCTGCCGTTTCACGGCTAGAAAAAGCATTGATCACAGGCAAACCATCACCATCTATTGCCACATTGCAA
AAATATGCTGCGGCAATTGGTAAACATGTTGAAGTGCGTTTTGTCTAA

Structures

Toxin

• AlphaFold DB: S3T2E4