Detailed information of TA system TA076564

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5791903..5792498
• Replicon: chromosome
• Accession: NZ_CP021380
• Organism: Pseudomonas aeruginosa strain CCBH4851

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: JG10_RS27420
• Protein ID: WP_003113526.1
• Coordinates: 5792220..5792498 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JG10_RS27415
• Protein ID: WP_003113527.1
• Coordinates: 5791903..5792208 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JG10_RS27380	5787044..5787892	+	849	WP_003099284.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
JG10_RS27390	5788059..5789000	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
JG10_RS27395	5789117..5789731	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
JG10_RS27400	5789773..5790357	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
JG10_RS27405	5790398..5791498	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
JG10_RS27415	5791903..5792208	-	306	WP_003113527.1	HigA family addiction module antidote protein	Antitoxin
JG10_RS27420	5792220..5792498	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JG10_RS27425	5792827..5795055	+	2229	WP_003099265.1	TonB-dependent receptor	-
JG10_RS27430	5795125..5795772	-	648	WP_003095021.1	carbonate dehydratase	-
JG10_RS27435	5795834..5797072	-	1239	WP_003113524.1	dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T76564 WP_003113526.1 NZ_CP021380:c5792498-5792220 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T76564 NZ_CP021380:c5792498-5792220 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT76564 WP_003113527.1 NZ_CP021380:c5792208-5791903 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT76564 NZ_CP021380:c5792208-5791903 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3