Detailed information of TA system TA076562

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 5062551..5063232
• Replicon: chromosome
• Accession: NZ_CP021380
• Organism: Pseudomonas aeruginosa strain CCBH4851

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: JG10_RS23950
• Protein ID: WP_015503432.1
• Coordinates: 5062867..5063232 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JG10_RS23945
• Protein ID: WP_016561576.1
• Coordinates: 5062551..5062874 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JG10_RS23905	5058172..5059050	+	879	WP_003109435.1	hypothetical protein	-
JG10_RS23915	5059562..5060428	-	867	WP_023081971.1	hypothetical protein	-
JG10_RS23920	5060458..5060709	-	252	WP_124136459.1	hypothetical protein	-
JG10_RS23925	5060926..5061480	-	555	WP_074197746.1	tyrosine-type recombinase/integrase	-
JG10_RS23930	5061654..5061929	-	276	WP_004353146.1	hypothetical protein	-
JG10_RS23935	5061929..5062261	-	333	WP_016561578.1	hypothetical protein	-
JG10_RS23940	5062316..5062435	+	120	Protein_4737	IS5/IS1182 family transposase	-
JG10_RS23945	5062551..5062874	-	324	WP_016561576.1	XRE family transcriptional regulator	Antitoxin
JG10_RS23950	5062867..5063232	-	366	WP_015503432.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JG10_RS23955	5063496..5063738	-	243	WP_043884955.1	hypothetical protein	-
JG10_RS23960	5063945..5064217	+	273	WP_003085667.1	hypothetical protein	-
JG10_RS23965	5064236..5064661	-	426	WP_003163196.1	VOC family protein	-
JG10_RS23970	5064762..5065646	+	885	WP_019725847.1	LysR family transcriptional regulator	-
JG10_RS23975	5065619..5066572	-	954	WP_003085661.1	LysR family transcriptional regulator	-
JG10_RS23980	5066793..5067227	+	435	WP_003158601.1	RidA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-63)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13798.14 Da
• Isoelectric Point: 4.8219

>T76562 WP_015503432.1 NZ_CP021380:c5063232-5062867 [Pseudomonas aeruginosa]
VAWDIEYTDEFGDWWGSLSEDEQESLAVTVRLLEERGPSLGHPHSSGINGSRPGHMRELRTQHGGRPFRTLYAFDPRRSA
ILPIGGDKTGDDRWYELNVPIADRLHDEHLHQLREEGLIDG

Nucleotide

• Length: 366 bp

>T76562 NZ_CP021380:c5063232-5062867 [Pseudomonas aeruginosa]
GTGGCGTGGGATATCGAGTACACCGACGAGTTCGGTGATTGGTGGGGTTCTCTGTCCGAGGATGAGCAGGAGTCGCTAGC
TGTGACGGTTCGCTTGCTTGAGGAGCGGGGGCCATCCTTGGGGCACCCCCACAGCAGCGGCATCAATGGCTCCCGCCCTG
GCCATATGAGAGAGCTACGGACTCAACACGGCGGGCGACCTTTCCGAACGCTTTACGCGTTTGACCCCAGACGCTCCGCG
ATACTGCCGATCGGTGGCGACAAGACTGGCGATGATCGTTGGTACGAATTGAACGTGCCCATTGCCGACCGCCTCCATGA
CGAACACTTGCACCAGCTCCGTGAGGAGGGACTGATCGATGGCTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11903.72 Da
• Isoelectric Point: 5.8900

>AT76562 WP_016561576.1 NZ_CP021380:c5062874-5062551 [Pseudomonas aeruginosa]
MAKKFSDLRAQMSPEAQARVEAKAQELLAEMPLNELRQSRGLSQKMLSEVLQVQQPAIAKMERRTDMYISTLRSHIEAMG
GQLEVIARFPDGAVKISNFADIEEAGA

Nucleotide

• Length: 324 bp

>AT76562 NZ_CP021380:c5062874-5062551 [Pseudomonas aeruginosa]
ATGGCTAAGAAATTCTCCGACCTTCGGGCGCAAATGTCGCCTGAGGCACAGGCCCGCGTCGAGGCCAAGGCCCAGGAACT
GTTGGCTGAAATGCCCCTGAATGAGTTGCGCCAGTCTAGAGGCTTGTCGCAGAAGATGCTGTCTGAAGTGCTGCAGGTGC
AGCAGCCGGCAATTGCCAAGATGGAGCGGCGTACGGATATGTACATCTCCACCTTGCGCAGCCACATCGAAGCCATGGGC
GGCCAGCTGGAAGTGATTGCACGCTTCCCGGATGGTGCGGTGAAAATCAGCAACTTCGCGGATATCGAAGAGGCGGGCGC
CTAA