Detailed information of TA system TA076536

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 6377499..6378135
• Replicon: chromosome
• Accession: NZ_CP021354
• Organism: Rhodococcus sp. S2-17

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: CBI38_RS29600
• Protein ID: WP_109334562.1
• Coordinates: 6377499..6377780 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CBI38_RS29605
• Protein ID: WP_109334564.1
• Coordinates: 6377842..6378135 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CBI38_RS29570	6372699..6373442	-	744	WP_109334554.1	hypothetical protein	-
CBI38_RS29575	6373768..6373971	-	204	WP_109334556.1	hypothetical protein	-
CBI38_RS29580	6374421..6374924	+	504	WP_109334558.1	hypothetical protein	-
CBI38_RS29585	6374933..6375658	-	726	WP_109335464.1	hypothetical protein	-
CBI38_RS29595	6376127..6377095	-	969	WP_109334560.1	hypothetical protein	-
CBI38_RS29600	6377499..6377780	+	282	WP_109334562.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CBI38_RS29605	6377842..6378135	+	294	WP_109334564.1	HigA family addiction module antidote protein	Antitoxin
CBI38_RS29615	6378834..6380549	+	1716	WP_109334568.1	cutinase family protein	-
CBI38_RS29620	6380562..6382826	-	2265	WP_162603304.1	acyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-95)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10973.50 Da
• Isoelectric Point: 9.9047

>T76536 WP_109334562.1 NZ_CP021354:6377499-6377780 [Rhodococcus sp. S2-17]
VIESFADKETERVFRRQFSKKLPQGIQRIARRRLLLIEAATDVNDLRVPPGNRLEKLSGDRMGQYSVRINDQYRICFTWR
ATAAYDVEITDYH

Nucleotide

• Length: 282 bp

>T76536 NZ_CP021354:6377499-6377780 [Rhodococcus sp. S2-17]
GTGATCGAATCGTTCGCCGACAAAGAGACCGAGCGGGTCTTTCGCCGGCAGTTCTCGAAGAAGCTCCCGCAGGGCATTCA
GCGGATCGCGCGCCGGCGCCTCCTGTTAATCGAGGCGGCCACCGATGTCAACGACCTGCGGGTGCCCCCAGGCAACCGCC
TGGAGAAGCTATCCGGGGACCGGATGGGCCAGTACAGCGTTCGGATCAACGATCAGTACCGTATCTGTTTCACCTGGCGC
GCCACCGCCGCATACGACGTGGAGATCACCGACTATCACTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10817.46 Da
• Isoelectric Point: 9.9914

>AT76536 WP_109334564.1 NZ_CP021354:6377842-6378135 [Rhodococcus sp. S2-17]
MTTDLSPIHPGEILREDFLEPFGITQNRLATIIGVPPRRINEIVLGKRSITADTALRLAKAFGNSAQFWLNIQNRYELDR
AADKLGDTLAKITRLSA

Nucleotide

• Length: 294 bp

>AT76536 NZ_CP021354:6377842-6378135 [Rhodococcus sp. S2-17]
ATGACTACAGACCTGAGCCCCATTCACCCCGGTGAGATCCTCCGTGAGGACTTCCTCGAGCCGTTCGGCATCACGCAGAA
CCGCTTAGCCACGATCATCGGTGTTCCGCCCCGACGGATCAACGAGATCGTGCTCGGAAAGCGGTCGATCACCGCAGACA
CCGCGTTGCGGCTGGCCAAGGCGTTCGGCAATAGTGCGCAGTTCTGGCTCAATATCCAGAACCGCTACGAGCTCGACCGT
GCGGCCGACAAGCTAGGCGACACCCTCGCCAAGATCACGCGTCTCTCGGCATAG