Detailed information of TA system TA076534

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 19484..20069
• Replicon: plasmid pB8300
• Accession: NZ_CP021348
• Organism: Acinetobacter baumannii strain B8300

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A3F3L0E6
• Locus tag: AB987_RS18750
• Protein ID: WP_002126330.1
• Coordinates: 19484..19804 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AB987_RS18755
• Protein ID: WP_002126333.1
• Coordinates: 19797..20069 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AB987_RS18730	15214..16143	+	930	WP_000200914.1	replication initiation protein RepM	-
AB987_RS18735	16207..16773	+	567	WP_000858429.1	plasmid replication DNA-binding protein	-
AB987_RS18740	17049..17276	+	228	WP_049590607.1	hypothetical protein	-
AB987_RS18745	17660..19183	-	1524	WP_049590608.1	TerC family protein	-
AB987_RS18750	19484..19804	+	321	WP_002126330.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AB987_RS18755	19797..20069	+	273	WP_002126333.1	helix-turn-helix domain-containing protein	Antitoxin
AB987_RS18760	20507..21672	+	1166	WP_085942227.1	IS3-like element ISAba22 family transposase	-
AB987_RS18765	21623..22150	-	528	WP_049590609.1	hypothetical protein	-
AB987_RS18770	22157..23287	-	1131	WP_000085720.1	DUF262 domain-containing protein	-
AB987_RS18775	23422..23613	-	192	WP_002125028.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	katA	1..25150	25150

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12191.98 Da
• Isoelectric Point: 7.9834

>T76534 WP_002126330.1 NZ_CP021348:19484-19804 [Acinetobacter baumannii]
MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDKGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVNEDDQIFFLVAY
PKSVKDNLTDKETAILRQLVKEQFHG

Nucleotide

• Length: 321 bp

>T76534 NZ_CP021348:19484-19804 [Acinetobacter baumannii]
ATGTTATTCATTGAAACCAGTATCTTTACCAAGCAAATCAAAGACCTTGTATCTGATGAGGAATATCGTCAACTTCAACA
AGATCTATTGGTTCAGCCAGATAAAGGTGACTTAATCAAGAATGGCGGTGGCATTCGAAAAGTACGTTGTGCTCAAGGGA
ATAAAGGCAAAAGTGGCGGTATCAGAGTAATTTACTATTGGGTAAATGAGGATGATCAAATCTTTTTCTTGGTGGCTTAT
CCAAAATCTGTAAAAGATAATCTAACGGATAAAGAAACCGCCATTCTGCGCCAACTCGTGAAGGAGCAATTTCATGGATA
A

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10177.62 Da
• Isoelectric Point: 9.8622

>AT76534 WP_002126333.1 NZ_CP021348:19797-20069 [Acinetobacter baumannii]
MDNNLFDDLVASIKEAGAIKRKEIQASRTTELKLPDIKEVREKTGLSQSEFAARLHISHRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT76534 NZ_CP021348:19797-20069 [Acinetobacter baumannii]
ATGGATAACAACTTATTTGATGACTTAGTTGCTTCGATTAAAGAAGCAGGAGCAATCAAGCGCAAAGAGATCCAAGCTAG
TCGAACGACCGAACTTAAATTACCAGATATTAAGGAAGTGCGAGAAAAAACTGGTTTAAGTCAAAGCGAATTTGCAGCAC
GTCTCCACATTAGCCATCGTACTCTGCAAAACTGGGAACAAGGCCGACGCTATCCAACAGGACCAGCAGCAACATTGATT
CGAATTTTAGATGCACATCCTAGTCTTATTTAA

Structures

Toxin

• AlphaFold DB: A0A3F3L0E6