Detailed information of TA system TA076533

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 7416..8001
• Replicon: plasmid pB8300
• Accession: NZ_CP021348
• Organism: Acinetobacter baumannii strain B8300

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N8ZPD7
• Locus tag: AB987_RS18680
• Protein ID: WP_000897309.1
• Coordinates: 7416..7736 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AB987_RS18685
• Protein ID: WP_005086343.1
• Coordinates: 7729..8001 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AB987_RS18650	3701..4228	+	528	WP_002124964.1	plasmid pRiA4b ORF-3 family protein	-
AB987_RS18655	4458..4769	+	312	WP_005086328.1	BrnT family toxin	-
AB987_RS18660	4732..5046	+	315	WP_005086327.1	BrnA antitoxin family protein	-
AB987_RS18665	5141..5668	+	528	WP_000537572.1	plasmid pRiA4b ORF-3 family protein	-
AB987_RS18670	5849..6460	+	612	WP_004740252.1	recombinase family protein	-
AB987_RS18675	6535..7188	-	654	WP_005086345.1	hypothetical protein	-
AB987_RS18680	7416..7736	+	321	WP_000897309.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AB987_RS18685	7729..8001	+	273	WP_005086343.1	helix-turn-helix domain-containing protein	Antitoxin
AB987_RS18690	8496..9101	-	606	WP_005086330.1	DUF4326 domain-containing protein	-
AB987_RS18695	9104..9544	-	441	WP_005086332.1	DUF488 domain-containing protein	-
AB987_RS18700	9558..10184	-	627	WP_005086335.1	hypothetical protein	-
AB987_RS18705	10359..10583	-	225	WP_002072934.1	hypothetical protein	-
AB987_RS18710	10732..10974	+	243	WP_001015364.1	hypothetical protein	-
AB987_RS18715	11047..12246	-	1200	WP_002072939.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	katA	1..25150	25150

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12209.01 Da
• Isoelectric Point: 7.9835

>T76533 WP_000897309.1 NZ_CP021348:7416-7736 [Acinetobacter baumannii]
MLFIETSIFTKQIKELVSDEEYRQLQQDLLVQPDKGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETSILRQLVKEQFHG

Nucleotide

• Length: 321 bp

>T76533 NZ_CP021348:7416-7736 [Acinetobacter baumannii]
ATGTTATTTATTGAAACCAGTATTTTTACCAAGCAAATTAAAGAGCTTGTGAGTGATGAAGAATATCGTCAGCTTCAGCA
AGATCTCTTGGTACAGCCCGATAAAGGCGACTTAATCAAGAATGGCGGTGGTATTCGTAAAGTACGTTGTGCTCAAGGTA
ACAAGGGTAAAAGTGGTGGTATCCGGGTGATCTATTATTGGGTCACAGAGGATGATCAGATCTTTTTCCTGGTGGCTTAT
CCAAAGTCTGTCAAAGATAATCTGACAGACAAAGAGACCTCGATCCTACGTCAATTAGTGAAGGAGCAATTTCATGGATA
A

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10094.53 Da
• Isoelectric Point: 7.2045

>AT76533 WP_005086343.1 NZ_CP021348:7729-8001 [Acinetobacter baumannii]
MDNNLFDDLVASIKEAGAIKRNEIKASRVTELELPDIKEVREKTGLTQAEFAARLHISARTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT76533 NZ_CP021348:7729-8001 [Acinetobacter baumannii]
ATGGATAACAACTTATTTGATGACCTGGTTGCTTCAATTAAAGAAGCTGGGGCCATCAAACGTAATGAGATAAAGGCAAG
CCGCGTGACTGAGCTTGAATTGCCGGATATTAAAGAAGTACGTGAGAAAACTGGCTTAACCCAGGCAGAATTCGCCGCAC
GTTTGCATATCAGCGCGAGAACCCTACAAAACTGGGAACAAGGCAGACGCTATCCAACAGGGCCAGCAGCAACCTTGATT
CGTATTCTAGATGCGCATCCAAGCCTGATTTAA

Structures

Toxin

• AlphaFold DB: A0A855WJQ3