Detailed information of TA system TA076532

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3357107..3357758
• Replicon: chromosome
• Accession: NZ_CP021347
• Organism: Acinetobacter baumannii strain B8300

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S3SZA7
• Locus tag: AB987_RS16280
• Protein ID: WP_000269903.1
• Coordinates: 3357107..3357463 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AB987_RS16285
• Protein ID: WP_001140619.1
• Coordinates: 3357456..3357758 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AB987_RS16270	3352396..3354675	-	2280	WP_049590968.1	excinuclease ABC subunit UvrA	-
AB987_RS16275	3355232..3356557	+	1326	Protein_3134	ISNCY family transposase	-
AB987_RS16280	3357107..3357463	+	357	WP_000269903.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AB987_RS16285	3357456..3357758	+	303	WP_001140619.1	XRE family transcriptional regulator	Antitoxin
AB987_RS19045	3357751..3357921	+	171	WP_171059625.1	hypothetical protein	-
AB987_RS16290	3357949..3358881	+	933	WP_004739252.1	IS5-like element ISAba13 family transposase	-
AB987_RS16295	3359463..3360614	-	1152	WP_000221973.1	trypsin-like peptidase domain-containing protein	-
AB987_RS16300	3360634..3361444	-	811	Protein_3140	M48 family metalloprotease	-
AB987_RS16305	3361431..3361919	-	489	WP_038342305.1	phosphate-starvation-inducible PsiE family protein	-
AB987_RS16310	3361931..3362179	-	249	WP_002084120.1	diguanylate cyclase	-
AB987_RS16320	3362322..3362673	+	352	Protein_3143	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	3351434..3358881	7447

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (13-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13486.45 Da
• Isoelectric Point: 7.1645

>T76532 WP_000269903.1 NZ_CP021347:3357107-3357463 [Acinetobacter baumannii]
MWTVITTDLFNEWLAQQDQSTQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKGGKKRFYKEMLDIADQQYEIHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T76532 NZ_CP021347:3357107-3357463 [Acinetobacter baumannii]
ATGTGGACAGTCATTACAACGGATCTGTTTAACGAATGGCTTGCACAGCAAGATCAATCTACACAAGAGAAAGTACTCGC
AGCGTTGGTCGTTCTACAGCAACAGGGACCAAGTTTAGGTCGTCCTTTAGTAGATACCGTCTATGACTCCAAATTTACCA
ATATGAAAGAGCTACGTGTTCAGCATCGAGGTAAACCACTGAGAGCCTTTTTCGCGTTTGATCCTTTACGACAAGCTATT
GTGCTGTGTATCGGTGATAAAGGCGGTAAAAAGCGTTTTTATAAAGAGATGTTAGATATTGCCGATCAGCAATACGAAAT
TCATCTTTCAACTTTAGGAGATCAATCTAATGGCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11206.01 Da
• Isoelectric Point: 6.7282

>AT76532 WP_001140619.1 NZ_CP021347:3357456-3357758 [Acinetobacter baumannii]
MAKTLQELLANRSPESQARIQQMADELLLETQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT76532 NZ_CP021347:3357456-3357758 [Acinetobacter baumannii]
ATGGCTAAGACCCTACAAGAATTATTGGCTAATCGCTCTCCAGAGAGTCAAGCTCGTATTCAACAAATGGCGGATGAATT
ATTGTTGGAAACTCAGCTTCACCTTATTCGTGAAGAGTTAGAGATTTCTCAAAAAGAACTGGCTGCAACCCTTGGAATCA
AACAGCCTTCATTGTCAGCAATCGAAAATCGTGGTCATGATCTTAAAATTTCAACGATGAAAAAGTATGTCGAAGCAATG
GGCGGAAAACTTCGTATTGATGTAGAGCTTCCAACAGGAAAACACATAGGATTCAATGTCTAA

Structures

Toxin

• AlphaFold DB: A0A836MG83