Detailed information of TA system TA076522

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 27266..27692
• Replicon: plasmid p95NR1B
• Accession: NZ_CP021341
• Organism: Escherichia coli strain 95NR1

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: EC95NR1_RS30070
• Protein ID: WP_001312861.1
• Coordinates: 27266..27424 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	27468..27692 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EC95NR1_RS30030	22637..23326	-	690	WP_000283373.1	conjugal transfer transcriptional regulator TraJ	-
EC95NR1_RS30035	23513..23896	-	384	WP_001151524.1	relaxosome protein TraM	-
EC95NR1_RS30040	24310..24819	+	510	Protein_23	transglycosylase SLT domain-containing protein	-
EC95NR1_RS30045	25116..25937	-	822	WP_001234469.1	DUF945 domain-containing protein	-
EC95NR1_RS30050	26057..26344	-	288	WP_000107535.1	hypothetical protein	-
EC95NR1_RS31325	26700..26912	+	213	WP_072002095.1	hypothetical protein	-
EC95NR1_RS30070	27266..27424	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	27468..27692	-	225	NuclAT_0	-	Antitoxin
-	27468..27692	-	225	NuclAT_0	-	Antitoxin
-	27468..27692	-	225	NuclAT_0	-	Antitoxin
-	27468..27692	-	225	NuclAT_0	-	Antitoxin
EC95NR1_RS31035	27504..27692	+	189	WP_001299721.1	hypothetical protein	-
EC95NR1_RS30075	27704..28423	-	720	WP_001276234.1	plasmid SOS inhibition protein A	-
EC95NR1_RS30080	28420..28854	-	435	WP_000845949.1	conjugation system SOS inhibitor PsiB	-
EC95NR1_RS30085	28909..30867	-	1959	WP_001145473.1	ParB/RepB/Spo0J family partition protein	-
EC95NR1_RS30090	30926..31159	-	234	WP_000006004.1	DUF905 domain-containing protein	-
EC95NR1_RS30095	31215..31742	-	528	WP_000290793.1	single-stranded DNA-binding protein	-
EC95NR1_RS30115	32215..32475	-	261	WP_071600428.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	espP / hlyC / hlyA / hlyB / hlyD	1..81853	81853

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T76522 WP_001312861.1 NZ_CP021341:c27424-27266 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T76522 NZ_CP021341:c27424-27266 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT76522 NZ_CP021341:c27692-27468 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin