Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2494339..2494841 Replicon chromosome
Accession NZ_CP021339
Organism Escherichia coli strain 95NR1

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag EC95NR1_RS13660 Protein ID WP_000767829.1
Coordinates 2494339..2494593 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag EC95NR1_RS13665 Protein ID WP_001259255.1
Coordinates 2494590..2494841 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EC95NR1_RS13635 2489354..2489581 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
EC95NR1_RS13640 2489595..2490653 - 1059 WP_000492340.1 transport protein YeeE -
EC95NR1_RS13645 2490832..2492190 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
EC95NR1_RS30715 2492180..2492242 - 63 WP_010723108.1 membrane protein YoeI -
EC95NR1_RS13650 2492457..2493386 - 930 WP_000803331.1 LysR family transcriptional regulator -
EC95NR1_RS13655 2493432..2494256 - 825 WP_000754755.1 SDR family oxidoreductase -
EC95NR1_RS13660 2494339..2494593 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
EC95NR1_RS13665 2494590..2494841 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
EC95NR1_RS13670 2495125..2495175 + 51 WP_001364200.1 his operon leader peptide -
EC95NR1_RS13680 2495321..2496220 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
EC95NR1_RS13685 2496226..2497530 + 1305 WP_001345258.1 histidinol dehydrogenase -
EC95NR1_RS13690 2497527..2498606 + 1080 WP_000102292.1 histidinol-phosphate transaminase -
EC95NR1_RS13695 2498603..2499670 + 1068 WP_000080054.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T76511 WP_000767829.1 NZ_CP021339:c2494593-2494339 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T76511 NZ_CP021339:c2494593-2494339 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT76511 WP_001259255.1 NZ_CP021339:c2494841-2494590 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT76511 NZ_CP021339:c2494841-2494590 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References